Department of Electronic Materials Engineering, Kwangwoon University, Seoul 139-701, Republic of Korea.
Biosensors (Basel). 2023 May 3;13(5):516. doi: 10.3390/bios13050516.
Dopamine is a catecholamine neurotransmitter that plays a significant role in the human central nervous system, even at extremely low concentrations. Several studies have focused on rapid and accurate detection of dopamine levels using field-effect transistor (FET)-based sensors. However, conventional approaches have poor dopamine sensitivity with values <11 mV/log [DA]. Hence, it is necessary to increase the sensitivity of FET-based dopamine sensors. In the present study, we proposed a high-performance dopamine-sensitive biosensor platform based on dual-gate FET on a silicon-on-insulator substrate. This proposed biosensor overcame the limitations of conventional approaches. The biosensor platform consisted of a dual-gate FET transducer unit and a dopamine-sensitive extended gate sensing unit. The capacitive coupling between the top- and bottom-gate of the transducer unit allowed for self-amplification of the dopamine sensitivity, resulting in an increased sensitivity of 373.98 mV/log[DA] from concentrations 10 fM to 1 μM. Therefore, the proposed FET-based dopamine sensor is expected to be widely applied as a highly sensitive and reliable biosensor platform, enabling fast and accurate detection of dopamine levels in various applications such as medical diagnosis and drug development.
多巴胺是一种儿茶酚胺神经递质，在人类中枢神经系统中发挥着重要作用，即使在极低的浓度下也是如此。已有多项研究致力于使用场效应晶体管（FET）为基础的传感器来快速准确地检测多巴胺水平。然而，传统方法的多巴胺灵敏度较差，其值<11 mV/log[DA]。因此，有必要提高基于 FET 的多巴胺传感器的灵敏度。在本研究中，我们提出了一种基于绝缘体上硅的双栅 FET 的高性能多巴胺敏感生物传感器平台。该生物传感器克服了传统方法的局限性。该生物传感器平台由双栅 FET 换能器单元和多巴胺敏感扩展栅感应单元组成。换能器单元的顶栅和底栅之间的电容耦合允许多巴胺灵敏度的自放大，从而使从 10 fM 到 1 μM 的浓度范围内的灵敏度提高到 373.98 mV/log[DA]。因此，所提出的基于 FET 的多巴胺传感器有望作为一种高灵敏度和可靠的生物传感器平台得到广泛应用，能够快速准确地检测各种应用中的多巴胺水平，如医疗诊断和药物开发。
