Matsishina Nathalia Valerievna, Ermak Marina Vladimirovna, Kim Irina Vyacheslavovna, Fisenko Petr Viktorovich, Sobko Olga Abdulalievna, Klykov Alexey Grigorievich, Emel'yanov Alexey Nikolaevich
FSBSI «FSC of Agricultural Biotechnology of the Far East Named after A.K. Chaiki», Timiryazevsky stl., Volozhenina st., 30 B, 692539 Ussuriysk, Russia.
Insects. 2023 May 13;14(5):459. doi: 10.3390/insects14050459.
is an intrinsic element in the agroecosystem of potato fields. The issues of relationships in the system "potato ladybird beetle-potato plant" have not yet been studied. To study the effect of potato varieties on the potato ladybird beetle, only hatched and active larvae with a hatching rate close to 100% were selected from a laboratory colony. Larvae of the first summer generation collected in potato fields were used in our study to determine the level of adrenaline in the bodies of insects, fresh potato leaves were used to study the content of glycoalkaloids, the content and activity of proteinase inhibitors. The larvae that fed on plants of varieties Belmonda, Queen Anne, Lilly, Dachny, Kazachok, Yubilyar, and Avgustin demonstrated the highest level of stress while the stress level in those that fed on variety Smak was the lowest. The damage inflicted by potato ladybird beetles on leaves of some studied potato varieties led to a progressive increase in the content of glycoalkaloids already within 24 h after the phytophages had been transferred. In most cases, the content of glycoalkoloids increased by 20% within five days. Potato ladybird beetles feeding on plants of different potato varieties caused a progressive increase in proteinase inhibitors (% of the control). Plants of variety Smak did not show a significant increase in the content of alkaloids in the herbage in response to the damage. A correlation was established between the mortality rate, the activity of proteinase inhibitors, the dynamics of glycoalkaloids, and the level of adrenaline, which could be formulated as follows: the higher the content of glycoalkaloids and the activity of proteinase inhibitors in the tissues of potato plants, the higher the level of stress in the potato ladybird beetles that feed on them.
是马铃薯田农业生态系统中的一个内在要素。“马铃薯瓢虫 - 马铃薯植株”系统中的关系问题尚未得到研究。为了研究马铃薯品种对马铃薯瓢虫的影响，仅从实验室种群中挑选孵化率接近100%的刚孵化且活跃的幼虫。我们的研究使用在马铃薯田中采集的第一代夏季幼虫来测定昆虫体内肾上腺素水平，用新鲜马铃薯叶来研究糖苷生物碱含量、蛋白酶抑制剂的含量和活性。取食品种为Belmonda、Queen Anne、Lilly、Dachny、Kazachok、Yubilyar和Avgustin植株的幼虫表现出最高的应激水平，而取食Smak品种的幼虫应激水平最低。马铃薯瓢虫对一些研究的马铃薯品种叶片造成的损害，在植食性昆虫转移后24小时内就导致糖苷生物碱含量逐渐增加。在大多数情况下，五天内糖苷生物碱含量增加了20%。取食不同马铃薯品种植株的马铃薯瓢虫导致蛋白酶抑制剂（相对于对照的百分比）逐渐增加。Smak品种的植株在受到损害后，其牧草中的生物碱含量没有显著增加。死亡率、蛋白酶抑制剂活性、糖苷生物碱动态变化和肾上腺素水平之间建立了一种相关性，可表述如下：马铃薯植株组织中糖苷生物碱含量和蛋白酶抑制剂活性越高，取食它们的马铃薯瓢虫的应激水平就越高。
