Liu Yijun, Mai Biyi, Li Zhiyun, Feng Xingqin, Chen Yunlan, Lin Lijing, Xia Qiuyu
Hainan Key Laboratory of Storage & Processing of Fruits and Vegetables, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, No. 48 Renmindadaonan, Zhanjiang 524001, China.
College of Food Science and Technology, Guangdong Ocean University, No. 1 Haida Road, Mazhang District, Zhanjiang 524088, China.
J Fungi (Basel). 2023 Apr 26;9(5):514. doi: 10.3390/jof9050514.
Various carbon sources affect the growth of the fruiting body, and the cassava stalk is considered a promising carbon source for . The composition, functional group characteristics, molecular weight distribution, antioxidant activity in vitro, and growth effect of LGG of polysaccharides (GLPs) under cassava stalk stress were investigated by gas chromatography-mass spectrometry, near-infrared spectroscopy, and gel chromatography. The results showed that GLPs consisted of D-glucose, D-galactose, and seven other monosaccharides. The end of the sugar chain had β-D-Glc and β-D-Gal configurations. The total sugar content in GLP1 was the highest (4.07%), and GLP1, GLP2, GLP3, and GLP5 had the β-D-Gal configuration, while GLP4 and GLP6 had the β-D-Glc configuration. The greater the proportion of cassava stalk, the greater the maximum molecular weight of GLPs. The total antioxidant capacities of GLPs obtained from different cassava stalks significantly varied, as well as their stimulating effects on the LGG growth. Higher concentrations of GLPs corresponded to the more intensive growth of LGG. This study provided essential data support for cassava stalk as a carbon source in cultivation.
多种碳源会影响子实体的生长,木薯秸秆被认为是一种很有前景的碳源。通过气相色谱 - 质谱联用、近红外光谱和凝胶色谱法研究了木薯秸秆胁迫下多糖(GLPs)的组成、官能团特征、分子量分布、体外抗氧化活性以及对嗜酸乳杆菌(LGG)的生长影响。结果表明,GLPs由D - 葡萄糖、D - 半乳糖和其他七种单糖组成。糖链末端具有β - D - Glc和β - D - Gal构型。GLP1中的总糖含量最高(4.07%),GLP1、GLP2、GLP3和GLP5具有β - D - Gal构型,而GLP4和GLP6具有β - D - Glc构型。木薯秸秆所占比例越大,GLPs的最大分子量越大。从不同木薯秸秆中获得的GLPs的总抗氧化能力以及它们对LGG生长的刺激作用存在显著差异。GLPs浓度越高,LGG的生长越旺盛。本研究为木薯秸秆作为栽培中的碳源提供了重要的数据支持。