Yang Ji-Yun, Fang Yu-Lan, Wang Ping, Ye Jian-Ren, Huang Lin
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Front Microbiol. 2018 Oct 24;9:2311. doi: 10.3389/fmicb.2018.02311. eCollection 2018.
Potassium has an important role to play in multiple cellular processes. In , the serine/threonine (S/T) kinase Sat4/Hal4 is required for potassium accumulation, and thus, regulates the resistance to sodium salts and helps in the stabilization of other plasma membrane transporters. However, the functions of Sat4 in filamentous phytopathogenic fungi are largely unknown. In this study, ChSat4, the yeast Sat4p homolog in , has been identified. Target deletion of resulted in defects in mycelial growth and sporulation. Intracellular K accumulation was significantly decreased in the deletion mutant. Additionally, the Δ mutant showed defects in cell wall integrity, hyperoxide stress response, and pathogenicity. Localization pattern analysis indicated ChSat4 was localized in the cytoplasm. Furthermore, ChSat4 showed high functional conservation with the homolog FgSat4 in . Taken together, our data indicated that ChSat4 was important for intracellular K accumulation and infection morphogenesis in .
钾在多种细胞过程中发挥着重要作用。在[具体生物名称]中，丝氨酸/苏氨酸（S/T）激酶Sat4/Hal4是钾积累所必需的，因此，它调节对钠盐的抗性，并有助于稳定其他质膜转运蛋白。然而，Sat4在丝状植物病原真菌中的功能在很大程度上尚不清楚。在本研究中，已鉴定出[具体生物名称]中酵母Sat4p的同源物ChSat4。[具体生物名称]中ChSat4的靶向缺失导致菌丝生长和孢子形成缺陷。缺失突变体中细胞内钾积累显著减少。此外，Δ突变体在细胞壁完整性、超氧化物应激反应和致病性方面表现出缺陷。定位模式分析表明ChSat4定位于细胞质中。此外，ChSat4与[具体生物名称]中的同源物FgSat4具有高度的功能保守性。综上所述，我们的数据表明ChSat4对[具体生物名称]中的细胞内钾积累和感染形态发生很重要。
