Yang Yong, Zhang Wanze, Zhang Zhanwei, Yang Ting, Xu Zhuo, Zhang Chuanbo, Guo Bing, Lu Wenyu
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
CNOOC EnerTech-Safety & Environmental Protection Co., Tianjin 300457, China.
Bioengineering (Basel). 2023 May 8;10(5):561. doi: 10.3390/bioengineering10050561.
In this article, we report a method for preparing an immobilized bacterial agent of petroleum-degrading bacteria W33 by combining high-density fermentation and bacterial immobilization technology and testing its bioremediation effect on petroleum-contaminated soil. After determining the optimal combination of MgCl, CaCl concentration, and culture time in the fermentation conditions by conducting a response surface analysis, the cell concentration reached 7.48 × 10 CFU/mL by 5 L fed-batch fermentation. The W33-vermiculite-powder-immobilized bacterial agent mixed with sophorolipids and rhamnolipids in a weight ratio of 9:10 was used for the bioremediation of petroleum-contaminated soil. After 45 days of microbial degradation, 56.3% of the petroleum in the soil with 20,000 mg/kg petroleum content was degraded, and the average degradation rate reached 250.2 mg/kg/d.
在本文中，我们报道了一种通过结合高密度发酵和细菌固定化技术制备石油降解菌W33固定化菌剂的方法，并测试其对石油污染土壤的生物修复效果。通过响应面分析确定发酵条件下MgCl、CaCl浓度和培养时间的最佳组合后，通过5L补料分批发酵，细胞浓度达到7.48×10CFU/mL。将重量比为9:10的W33-蛭石粉固定化菌剂与槐糖脂和鼠李糖脂混合，用于石油污染土壤的生物修复。经过45天的微生物降解，石油含量为20000mg/kg的土壤中56.3%的石油被降解，平均降解率达到250.2mg/kg/d。
