Vidaković Knežević Suzana, Knežević Slobodan, Vranešević Jelena, Kravić Sneẑana Ž, Lakićević Brankica, Kocić-Tanackov Sunčica, Karabasil Nedjeljko
Scientific Veterinary Institute "Novi Sad", 21000 Novi Sad, Serbia.
Faculty of Technology Novi Sad, University of Novi Sad, 21000 Novi Sad, Serbia.
Foods. 2023 May 9;12(10):1930. doi: 10.3390/foods12101930.
The composition of 18 essential oils was determined using gas chromatography-mass spectrometry, and their antilisterial activity was evaluated by the disk diffusion method, followed by the determination of the minimum inhibitory and minimum bactericidal concentrations. The most active essential oils were oregano, thyme, cinnamon, winter savory, and clove, with MIC values ranging from 0.09 to 1.78 µL/mL. We investigated the biofilm-forming potential of on polystyrene at 5 °C, 15 °C, and 37 °C in three different media. The formation of biofilm was found to be dependent on the temperature and the availability of nutrients. After treatment with selected essential oils, the reduction in biofilm biomass was in the range of 32.61% and 78.62%. Micromorphological changes in the treated by oregano and thyme essential oils were observed in the form of impaired cell integrity and cell lyses by using scanning electron microscope. Oregano and thyme essential oils (MIC and 2MIC) significantly ( < 0.05) reduced the population of in minced pork meat during storage at 4 °C. In conclusion, the obtained results indicated the good activity of some selected essential oils on , with bacteriostatic, bactericidal, and antibiofilm effects at very low concentrations.
采用气相色谱 - 质谱联用仪测定了18种精油的成分，并通过纸片扩散法评估了它们的抗李斯特菌活性，随后测定了最低抑菌浓度和最低杀菌浓度。活性最强的精油是牛至、百里香、肉桂、冬香薄荷和丁香，其最低抑菌浓度值在0.09至1.78微升/毫升之间。我们研究了在三种不同培养基中，该菌在5℃、15℃和37℃下在聚苯乙烯上形成生物膜的潜力。发现生物膜的形成取决于温度和营养物质的可用性。用选定的精油处理后，生物膜生物量的减少幅度在32.61%至78.62%之间。通过扫描电子显微镜观察发现，经牛至和百里香精油处理的该菌出现了细胞完整性受损和细胞裂解的微观形态变化。牛至和百里香精油（最低抑菌浓度和2倍最低抑菌浓度）在4℃储存期间显著（P<0.05）减少了碎猪肉中该菌的数量。总之，所得结果表明一些选定的精油对该菌具有良好的活性，在极低浓度下具有抑菌、杀菌和抗生物膜作用。
