Gwiazdowska Daniela, Waśkiewicz Agnieszka, Juś Krzysztof, Marchwińska Katarzyna, Frąk Szymon, Popowski Dominik, Pawlak-Lemańska Katarzyna, Uwineza Pascaline Aimee, Gwiazdowski Romuald, Padewska Daria, Roszko Marek, Bryła Marcin
Department of Natural Science and Quality Assurance, Institute of Quality Science, Poznań University of Economics and Business, Niepodległości 10, 61-875 Poznań, Poland.
Department of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland.
Molecules. 2024 Dec 10;29(24):5823. doi: 10.3390/molecules29245823.
Sustainable management of agri-food product safety presents a major challenge requiring extensive action to ensure food safety and consumer health. The pursuit of environmentally friendly solutions that will constitute an alternative to the chemical compounds commonly used in agriculture and the food industries is one of the most important problems. One solution is plant extracts containing various biologically active compounds and exhibiting antimicrobial activity. This study aims to determine the biological activity of extracts obtained from L. (leaves) by supercritical CO (SC-CO) extraction using different reaction conditions and compositions. In vitro studies revealed antimicrobial activity against selected bacteria (including Enteritidis, , and ) and fungi ( spp.), depending mainly on the microorganism species; however, extraction conditions also influenced these properties. The microscopic observations established by optical and fluorescence microscopy showed the changes in the fungal cell's viability and morphology. There was no observed significant release of intracellular material as stated based on ICP-MS analysis of sodium and potassium concentration. Antibiofilm properties of extract obtained by extraction at 40 °C were also demonstrated against , , and , with stronger properties observed against Gram-positive bacteria. Phytochemical characterization of the extracts was determined using a liquid chromatography system with an orbitrap mass spectrometer (LC/MS), identifying, i.e., phenolic acids: protocatechuic, hydroxybenzoic, caffeic, and rosmarinic; flavonoids: luteolin, naringenin, and kaempferol; and terpenoids: oleanolic and ursolic acids.
农业食品产品安全的可持续管理面临重大挑战，需要采取广泛行动以确保食品安全和消费者健康。寻求环保解决方案以替代农业和食品工业中常用的化合物是最重要的问题之一。一种解决方案是含有各种生物活性化合物并具有抗菌活性的植物提取物。本研究旨在通过使用不同反应条件和成分的超临界CO₂（SC-CO₂）萃取法，测定从罗勒叶中获得的提取物的生物活性。体外研究表明，其对选定的细菌（包括肠炎沙门氏菌、金黄色葡萄球菌和大肠杆菌）和真菌（曲霉属）具有抗菌活性，这主要取决于微生物种类；然而，提取条件也会影响这些特性。通过光学显微镜和荧光显微镜进行的微观观察显示了真菌细胞活力和形态的变化。基于钠和钾浓度的电感耦合等离子体质谱（ICP-MS）分析表明，未观察到细胞内物质的显著释放。在40℃下提取得到的提取物对金黄色葡萄球菌、大肠杆菌和白色念珠菌也具有抗生物膜特性，对革兰氏阳性菌的特性更强。使用配备轨道阱质谱仪的液相色谱系统（LC/MS）对提取物进行了植物化学表征，鉴定出了酚酸类：原儿茶酸、对羟基苯甲酸、咖啡酸和迷迭香酸；黄酮类：木犀草素、柚皮素和山奈酚；以及萜类：齐墩果酸和熊果酸。
