Sangkatip Rittichai, Jongwuttanaruk Kaona, Sriseubsai Wipoo
Department of Industrial Engineering, School of Engineering, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Department of Industrial Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand.
Polymers (Basel). 2023 May 16;15(10):2322. doi: 10.3390/polym15102322.
Materials and manufacturing technologies are necessary for tissue engineering and developing temporary artificial extracellular matrices. In this study, scaffolds were fabricated from freshly synthesized titanate (NaTiO) and its precursor titanium dioxide and their properties were investigated. The scaffolds with improved properties were then mixed with gelatin to form a scaffold material using the freeze-drying technique. To determine the optimal composition for the compression test of the nanocomposite scaffold, a mixture design with three factors of gelatin, titanate, and deionized water was used. Then, the scaffold microstructures were examined by scanning electron microscopy (SEM) to determine the porosity of the nanocomposite scaffolds. The scaffolds were fabricated as a nanocomposite and determined their compressive modulus values. The results showed that the porosity of the gelatin/NaTiO nanocomposite scaffolds ranged from 67% to 85%. When the mixing ratio was 100:0, the degree of swelling was 22.98%. The highest swelling ratio of 85.43% was obtained when the freeze-drying technique was applied to the mixture of gelatin and NaTiO with a mixing ratio of 80:20. The specimens formed (gelatin:titanate = 80:20) exhibited a compressive modulus of 30.57 kPa. The sample with a composition of 15.10% gelatin, 2% NaTiO, and 82.9% DI water, processed by the mixture design technique, showed the highest yield of 30.57 kPa in the compression test.
材料和制造技术对于组织工程和开发临时人工细胞外基质至关重要。在本研究中，由新合成的钛酸盐（NaTiO）及其前体二氧化钛制备了支架，并对其性能进行了研究。然后，使用冷冻干燥技术将具有改进性能的支架与明胶混合以形成支架材料。为了确定纳米复合支架压缩试验的最佳组成，采用了包含明胶、钛酸盐和去离子水三个因素的混合设计。然后，通过扫描电子显微镜（SEM）检查支架微观结构，以确定纳米复合支架的孔隙率。将支架制成纳米复合材料并测定其压缩模量值。结果表明，明胶/NaTiO纳米复合支架的孔隙率在67%至85%之间。当混合比例为100:0时，溶胀度为22.98%。当对混合比例为80:20的明胶和NaTiO混合物应用冷冻干燥技术时，获得了最高溶胀率85.43%。形成的试样（明胶:钛酸盐 = 80:20）的压缩模量为30.57 kPa。通过混合设计技术处理的、由15.10%明胶、2% NaTiO和82.9%去离子水组成的样品在压缩试验中显示出最高屈服强度30.57 kPa。
