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在 SARS-CoV-2 感染中，参与 miRNA 生物发生的基因未下调。

Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection.

机构信息

Laboratoire de Virologie ULR3610, University Lille and CHU Lille, F-59000 Lille, France.

Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, F-59000 Lille, France.

出版信息

Viruses. 2023 May 16;15(5):1177. doi: 10.3390/v15051177.

DOI:10.3390/v15051177
PMID:37243263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10222706/
Abstract

miRNAs, small non-coding RNAs that regulate gene expression, are involved in various pathological processes, including viral infections. Virus infections may interfere with the miRNA pathway through the inhibition of genes involved in miRNA biogenesis. A reduction in the number and the levels of miRNAs expressed in nasopharyngeal swabs of patients with severe COVID-19 was lately observed by us, pointing towards the potential of miRNAs as possible diagnostic or prognostic biomarkers for predicting outcomes among patients with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The objective of the present study was to investigate whether SARS-CoV-2 infection influences the expression levels of messenger RNAs (mRNAs) of key genes involved in miRNA biogenesis. mRNA levels of , , , , and Exportin-5 () were measured by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) in nasopharyngeal swab specimens from patients with COVID-19 and controls, as well as in cells infected with SARS-CoV-2 in vitro. Our data showed that the mRNA expression levels of , , , , and were not significantly different in patients with severe COVID-19 when compared to patients with non-severe COVID-19 and controls. Similarly, the mRNA expression of these genes was not affected by SARS-CoV-2 infection in NHBE and Calu-3 cells. However, in Vero E6 cells, , , , and mRNA levels were slightly upregulated 24 h after infection with SARS-CoV-2. In conclusion, we did not find evidence for downregulation of mRNA levels of miRNA biogenesis genes during SARS-CoV-2 infection, neither ex vivo nor in vitro.

摘要

miRNAs 是一种调节基因表达的小非编码 RNA，参与各种病理过程，包括病毒感染。病毒感染可能通过抑制 miRNA 生物发生相关基因来干扰 miRNA 途径。最近我们观察到，严重 COVID-19 患者鼻咽拭子中 miRNA 的数量和表达水平降低，表明 miRNA 作为可能的诊断或预后生物标志物，具有预测严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 感染患者结局的潜力。本研究旨在探讨 SARS-CoV-2 感染是否影响 miRNA 生物发生关键基因的信使 RNA (mRNA) 表达水平。通过定量逆转录聚合酶链反应 (RT-qPCR) 测量 COVID-19 患者和对照以及体外 SARS-CoV-2 感染细胞中鼻咽拭子标本中 、 、 、 、 和 Exportin-5 () 的 mRNA 水平。我们的数据表明，与非严重 COVID-19 患者和对照组相比，严重 COVID-19 患者的 、 、 、 和 mRNA 表达水平没有显著差异。同样，这些基因的 mRNA 表达不受 SARS-CoV-2 在 NHBE 和 Calu-3 细胞中的感染影响。然而，在 Vero E6 细胞中，SARS-CoV-2 感染 24 小时后， 、 、 、 和 mRNA 水平略有上调。总之，我们没有发现 SARS-CoV-2 感染期间 miRNA 生物发生基因的 mRNA 水平下调的证据，无论是在体外还是体内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b72b/10222706/cfbed7ffa199/viruses-15-01177-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b72b/10222706/2f974057f5e1/viruses-15-01177-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b72b/10222706/cfbed7ffa199/viruses-15-01177-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b72b/10222706/2f974057f5e1/viruses-15-01177-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b72b/10222706/95e8f280439e/viruses-15-01177-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b72b/10222706/d5c02bc0302e/viruses-15-01177-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b72b/10222706/98974d019150/viruses-15-01177-g004.jpg
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