School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China.
Mikrochim Acta. 2024 Nov 29;191(12):770. doi: 10.1007/s00604-024-06840-0.
A multienzyme-like L-argininemodified CeO (Arg-CeO) nanozyme was prepared for accurate total antioxidant capacity (TAC) determination of Ganoderma sichuanense (G. sichuanense). It exhibits oxidase-like and peroxidase-like activities catalyzing the generation of superoxide anion free radicals and hydroxyl radicals, accelerating 3, 3', 5, 5'-tetramethylbenzidine (TMB) chromogenic reaction. Antioxidants can effectively eliminate the generated free radicals, inhibiting the TMB chromogenic reaction. Hence, TAC can be assessed by Arg-CeO catalyzed TMB chromogenic reaction using ascorbic acid (AA) as a reference substance. TAC of G. sichuanense extracted using different solvents was determined, indicating the highest TAC observed in the G. sichuanense extracted by the mixture solvent (deionized water/ethanol/ethyl acetate = 1 : 1 : 1). A visual assessment platform based on Arg-CeO hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D printed device is established. TAC using AA as a reference substance (3.33 ~ 83.33 µM) can be detected with a detection limit as low as 0.58 µM. The platform can realize one-step quantitative determination of TAC in G. sichuanense within 40 min. The proposed visual assessment platform presented exhibits promising application prospects in the field of TAC assessment in food.
一种多酶样的 L-精氨酸修饰的 CeO（Arg-CeO）纳米酶被制备用于准确测定四川灵芝（G. sichuanense）的总抗氧化能力（TAC）。它表现出氧化酶样和过氧化物酶样活性，可催化超氧阴离子自由基和羟基自由基的生成，加速 3,3',5,5'-四甲基联苯胺（TMB）显色反应。抗氧化剂可以有效地消除生成的自由基，抑制 TMB 显色反应。因此，TAC 可以通过 Arg-CeO 催化的 TMB 显色反应，以抗坏血酸（AA）作为参比物质进行评估。测定了用不同溶剂提取的四川灵芝的 TAC，结果表明，在混合溶剂（去离子水/乙醇/乙酸乙酯=1:1:1）中提取的四川灵芝的 TAC 最高。建立了一种基于 Arg-CeO 水凝胶纳米酶的可视化评估平台，利用自编的智能手机应用程序和自行设计的 3D 打印设备。使用 AA 作为参比物质（3.33~83.33 µM）可以检测到 TAC，检测限低至 0.58 µM。该平台可以在 40 分钟内实现四川灵芝中 TAC 的一步定量测定。所提出的可视化评估平台在食品 TAC 评估领域具有广阔的应用前景。
