Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Asian Pac J Cancer Prev. 2023 May 1;24(5):1553-1560. doi: 10.31557/APJCP.2023.24.5.1553.
The aim of this study was to evaluate the expression alterations of CACS2 and its target gene, AKT, in T98G cell line treated with Temozolomide and Thiosemicarbazone complex (Ni, Cu) and to compare the results with each other.
Temozolomide and Thiosemicarbazone complexes were prepared in different concentrations. Cell culturing of T98G cell line was carried out and was classified into 3 groups based on the incubation time (24, 48, and 72h) with utilized agents, after RNA extraction the expression level of CACS2 and AKT genes were evaluated by Real-time PCR. Ultimately, the results were analyzed by Rest software.
CASC2 expression under Temozolomide treatment at different concentrations (100, 150, 200, and 250 µM) and different time periods (24, 48, and 72h) was increased. Moreover, its expression was significantly upregulated after treating with Ni at the concentrations of 100.5 and 104 µM after 24h. Furthermore, its expression was augmented after 72 h Cu treatment at the concentrations of 15, 16, 17, and 18 µM. In addition, AKT expression after Temozolomide and Thiosemicarbazone complex treatment was significantly decreased (P <0.001). The results showed that the expression alterations of CASC2 and its target gene, AKT, after treatment with Temozolomide and Thiosemicarbazone are highly depended on incubation time and concentration.
In a conclusion, the studied agents at different concentrations and times showed a high potential to control the expression of the studied lncRNA and gene in glioblastoma cells.
本研究旨在评估替莫唑胺和硫代卡巴肼配合物（Ni、Cu）处理 T98G 细胞系后 CACS2 及其靶基因 AKT 的表达变化，并将结果进行比较。
用不同浓度的替莫唑胺和硫代卡巴肼配合物进行细胞培养，根据孵育时间（24、48 和 72h）将 T98G 细胞系分为 3 组，提取 RNA 后，通过实时 PCR 评估 CACS2 和 AKT 基因的表达水平。最后，用 Rest 软件分析结果。
不同浓度（100、150、200 和 250μM）和不同时间（24、48 和 72h）替莫唑胺处理后 CASC2 的表达增加。此外，在 24h 时，100.5 和 104μM Ni 处理后，其表达显著上调。此外，在 15、16、17 和 18μM Cu 处理 72h 后，其表达增加。此外，替莫唑胺和硫代卡巴肼配合物处理后 AKT 的表达明显降低（P<0.001）。结果表明，替莫唑胺和硫代卡巴肼处理后 CASC2 及其靶基因 AKT 的表达变化高度依赖于孵育时间和浓度。
总之，不同浓度和时间的研究药物对胶质母细胞瘤细胞中研究 lncRNA 和基因的表达具有很大的控制潜力。
