National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
J Colloid Interface Sci. 2023 Oct;647:142-151. doi: 10.1016/j.jcis.2023.05.080. Epub 2023 May 18.
The biofilms formed by bacteria at the wound site can effectively protect the bacteria, which greatly weakens the effect of antibiotics. Herein, a microneedle patch for wound treatment is designed, which can effectively penetrate the biofilms in a physical way because of the penetration ability of the microneedles and the motion behavior of the nanomotors, and deliver bacterial quorum sensing inhibitor luteolin (Le) and nanomotors with multiple antibacterial properties within biofilms. Firstly, the nanomotors-loaded microneedle patches are prepared and characterized. The results of in vitro and in vivo experiments show that the microneedle patches have good biosafety and antibacterial properties. Among them, Le can inhibit the growth of biofilms. Further, under near-infrared (NIR) irradiation, the nanomotors loaded with photosensitizer ICG and nitric oxide (NO) donor L-arginine (L-Arg) can move in the biofilms under the double driving effect of photothermal and NO, and can give full play to the multiple anti-biological infection effects of photothermal therapy (PTT), photodynamic therapy (PDT) and NO, and finally realize the effective removal of biofilms and promote wound healing. The intervention of nanomotor technology has brought about a new therapeutic strategy for bacterial biofilm-related infection of wound.
细菌在伤口部位形成的生物膜可以有效地保护细菌，这大大降低了抗生素的效果。在此，设计了一种用于伤口治疗的微针贴片，由于微针的穿透能力和纳米马达的运动行为，它可以有效地以物理方式穿透生物膜，并在生物膜内输送群体感应抑制剂木樨草素（Le）和具有多种抗菌特性的纳米马达。首先，制备和表征了载纳米马达的微针贴片。体外和体内实验结果表明，微针贴片具有良好的生物安全性和抗菌性能。其中，Le 可以抑制生物膜的生长。此外，在近红外（NIR）照射下，负载光热剂吲哚菁绿（ICG）和一氧化氮（NO）供体 L-精氨酸（L-Arg）的纳米马达可以在生物膜下在光热和 NO 的双重驱动作用下移动，并能充分发挥光热治疗（PTT）、光动力治疗（PDT）和 NO 的多种抗生物感染作用，最终实现生物膜的有效去除和促进伤口愈合。纳米马达技术的介入为伤口细菌生物膜相关感染带来了新的治疗策略。
