Center of Hematology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Senior Department of Hematology, The Fifth Medical Center of PLA General Hospital, Beijing, China.
Immun Inflamm Dis. 2023 May;11(5):e853. doi: 10.1002/iid3.853.
Galectin-9 and myeloid-derived suppressor cells (MDSCs) have an important role in tumors, but their clinical values in chronic lymphocytic leukemia (CLL) have not been fully elucidated. This study aimed to analyze the prognosis values of Galectin-9 and MDSCs in CLL.
The concentrations of Galectin-9, argininase-1, and inducible nitric oxide synthase in serum were detected by enzyme-linked immune sorbent assay. The expression of Tim-3 protein in peripheral blood mononuclear cell was detected by Western blot. Flow cytometry was used to analyze the percentages of Tim-3 on T-cells (CD3 T, CD4 T, and CD8 T cells) and MDSCs.
Our results showed that Galectin-9 and MDSCs significantly increased in CLL patients and were closely related to the disease progression. Patient's receiver operating characteristic, progression-free survival, and Cox regression analysis showed that Galectin9 and MDSCs were poor prognostic factors of CLL.
Galectin-9 and MDSCs were associated with clinical progression and could be important prognostic indicators for CLL.
半乳糖凝集素-9 和髓系来源抑制细胞（MDSCs）在肿瘤中具有重要作用，但它们在慢性淋巴细胞白血病（CLL）中的临床价值尚未完全阐明。本研究旨在分析 Galectin-9 和 MDSCs 在 CLL 中的预后价值。
通过酶联免疫吸附试验检测血清中 Galectin-9、精氨酸酶-1 和诱导型一氧化氮合酶的浓度。通过 Western blot 检测外周血单个核细胞中 Tim-3 蛋白的表达。流式细胞术用于分析 T 细胞（CD3 T、CD4 T 和 CD8 T 细胞）和 MDSCs 上 Tim-3 的百分比。
我们的结果表明，Galectin-9 和 MDSCs 在 CLL 患者中显著增加，与疾病进展密切相关。患者的受试者工作特征曲线、无进展生存期和 Cox 回归分析表明，Galectin9 和 MDSCs 是 CLL 的不良预后因素。
Galectin-9 和 MDSCs 与临床进展相关，可能是 CLL 的重要预后指标。
