Hofmann H, Frisch-Niggemeyer W, Heinz F
J Gen Virol. 1979 Mar;42(3):505-11. doi: 10.1099/0022-1317-42-3-505.
An enzyme-linked immunosorbent assay was applied for determining separately IgM and IgG antibodies against tick-borne encephalitis virus. A micro-modification in microtitre plates proved to be at least as sensitive as the HI test. However, more precise information could be achieved by a macrotest using antigen coated polystyrene balls. False positive results in IgM antibody determinations could be caused by a rheumatoid factor. A high content of IgM antibodies in a serum could impair the determination of its IgG antibodies but not vice versa. Titres were expressed in comparison to a positive control serum.
采用酶联免疫吸附测定法分别检测抗蜱传脑炎病毒的IgM和IgG抗体。微量滴定板的微改良法被证明至少与血凝抑制试验一样灵敏。然而，使用包被抗原的聚苯乙烯球进行的宏观试验可获得更精确的信息。类风湿因子可能导致IgM抗体测定出现假阳性结果。血清中高含量的IgM抗体可能会影响其IgG抗体的测定，但反之则不然。滴度以与阳性对照血清比较的方式表示。
