使用酶标记抗原检测针对不同黄病毒的特异性免疫球蛋白M抗体。
Detection of specific immunoglobulin M antibody to different flaviviruses by use of enzyme-labeled antigens.
作者信息
Schmitz H, Emmerich P
出版信息
J Clin Microbiol. 1984 May;19(5):664-7. doi: 10.1128/jcm.19.5.664-667.1984.
Abstract
An enzyme immunoassay was developed for the detection of human immunoglobulin M (IgM) antibody to different flavivirus antigens. The IgM antibody of human sera was selectively bound to anti-IgM antibody-coated solid-phase plates. Flavivirus IgM antibodies were then detected by use of various enzyme-labeled antigens. The flavivirus antigens (dengue type 2 virus, West Nile virus, and tick-borne encephalitis virus) were produced in suckling mice. The antigens were labeled with horseradish peroxidase by adding the activated enzyme at alkaline pH to sucrose-acetone-treated antigens. Addition of unlabeled mouse brain suspension of uninfected animals to the diluted enzyme-labeled antigens effectively reduced nonspecific binding to the solid phase. In patients with acute flavivirus infections, viral IgM antibody could be demonstrated with high sensitivity. Furthermore, the enzyme-labeled antigen-IgM test showed greater specificity than the hemagglutination inhibition test.
摘要
开发了一种酶免疫测定法，用于检测针对不同黄病毒抗原的人免疫球蛋白M（IgM）抗体。人血清中的IgM抗体被选择性地结合到抗IgM抗体包被的固相板上。然后使用各种酶标记抗原检测黄病毒IgM抗体。黄病毒抗原（登革2型病毒、西尼罗河病毒和蜱传脑炎病毒）在乳鼠中产生。通过在碱性pH值下将活化酶添加到经蔗糖-丙酮处理的抗原中，用辣根过氧化物酶标记抗原。将未感染动物的未标记小鼠脑悬液添加到稀释的酶标记抗原中，有效地降低了与固相的非特异性结合。在急性黄病毒感染患者中，可高灵敏度地检测到病毒IgM抗体。此外，酶标记抗原-IgM试验比血凝抑制试验具有更高的特异性。
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