Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
Methods Mol Biol. 2023;2659:23-35. doi: 10.1007/978-1-0716-3159-1_2.
We are reporting on the utilization of high-throughput sequencing and different sequencing analysis tools to delineate identification of different isolates of the stripe rust fungal pathogen Puccinia striiformis f. sp. tritici (Pst). Different approaches are shown: utilization of rDNA sequences and random sequences that may be very useful to make sure that isolates belong to Pst and to distinguished closely related isolates. Identification of unique/lost sequences could lead to the identification of effectors associated with specific isolates.
我们报告了高通量测序和不同测序分析工具的利用，以描绘鉴定不同条锈菌真菌病原体小麦条锈菌（Pst）分离株的情况。展示了不同的方法：利用 rDNA 序列和随机序列，这可能非常有助于确保分离株属于 Pst 并区分密切相关的分离株。鉴定独特/丢失的序列可能导致与特定分离株相关的效应子的鉴定。
