Sørensen Chris K, Thach Tine, Hovmøller Mogens S
Department of Agroecology, Aarhus University, Flakkebjerg, Forsøgsvej 1, DK-4200 Slagelse, Denmark.
Plant Dis. 2016 Jun;100(6):1064-1070. doi: 10.1094/PDIS-12-15-1477-RE. Epub 2016 Mar 23.
The fungus Puccinia striiformis causes yellow (stripe) rust on wheat worldwide. In the present article, new methods utilizing an engineered fluid (Novec 7100) as a carrier of urediniospores were compared with commonly used inoculation methods. In general, Novec 7100 facilitated a faster and more flexible application procedure for spray inoculation and it gave highly reproducible results for virulence phenotyping. Six point inoculation methods were compared to find the most suitable for assessment of pathogen aggressiveness. The use of Novec 7100 and dry dilution with Lycopodium spores gave an inoculation success rate of 100% in two independent trials, which was significantly higher and more consistent than for spore suspension in Soltrol 170, water, water + Tween 20, and Noble agar + Tween 20. Both Soltrol 170 and Novec 7100 allowed precise quantification of inoculum, which is important for the assessment of quantitative epidemiological parameters. New protocols for spray and point inoculation of P. striiformis on wheat are presented, along with the prospect for applying these in rust research and resistance breeding activities.
条形柄锈菌在全球范围内引发小麦条锈病。在本文中，将利用一种工程流体（Novec 7100）作为夏孢子载体的新方法与常用接种方法进行了比较。总体而言，Novec 7100有助于喷雾接种的操作过程更快且更灵活，并且在毒力表型分析方面能给出高度可重复的结果。比较了六种点接种方法以找出最适合评估病原菌侵袭力的方法。在两项独立试验中，使用Novec 7100和与石松子孢子进行干稀释的接种成功率为100%，这显著高于并优于在Soltrol 170、水、水 + 吐温20以及Noble琼脂 + 吐温20中使用孢子悬浮液的接种成功率，且更为稳定。Soltrol 170和Novec 7100都能实现接种量的精确量化，这对于定量流行病学参数的评估很重要。本文介绍了条形柄锈菌在小麦上喷雾接种和点接种的新方案，以及将这些方案应用于锈病研究和抗性育种活动的前景。
