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利用 RNA 测序解析谷物中的植物-病原体相互作用。

Unraveling Plant-Pathogen Interactions in Cereals Using RNA-seq.

机构信息

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.

Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, Canada.

出版信息

Methods Mol Biol. 2023;2659:103-118. doi: 10.1007/978-1-0716-3159-1_9.

Abstract

Over the past two decades, there have been significant advancements in the realm of transcriptomics, or the study of genes and their expression. Modern RNA sequencing technologies and high-performance computing are creating a "big data" revolution that provides new opportunities to explore the interactions between cereals and pathogens that affect grain yield and food safety. These data are being used to annotate genes and gene variants, as well as identify differentially expressed genes and create global gene co-expression networks. Moreover, these data can unravel the complex interactions between pathogen and host and identify genes and pathways involved in these interactions. This information can then be used for disease mitigation and the development of crops with superior resistance.

摘要

在过去的二十年中,转录组学领域取得了重大进展,即研究基因及其表达。现代 RNA 测序技术和高性能计算正在引发一场“大数据”革命,为探索影响粮食产量和食品安全的谷物和病原体之间的相互作用提供了新的机会。这些数据正被用于注释基因和基因变异,识别差异表达基因并创建全局基因共表达网络。此外,这些数据可以揭示病原体和宿主之间的复杂相互作用,并鉴定参与这些相互作用的基因和途径。然后,这些信息可用于疾病缓解和开发具有卓越抗性的作物。

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