比较基因组和代谢组分析揭示了[具体物种1]和[具体物种2]之间的进化关系以及黄酮类生物合成。

Comparative genome and metabolome analyses uncover the evolution and flavonoid biosynthesis between and .

作者信息

Gao Gang, Abubakar Aminu Shehu, Chen Jikang, Chen Ping, Chen Kunmei, Yu Chunming, Wang Xiaofei, Qiu Xiaojun, Huang Xiaoyu, Shao Deyi, Wang Yue, Chen Yu, Zhu Aiguo

机构信息

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410221, People's Republic of China.

Department of Agronomy, Bayero University Kano, PMB 3011, Kano, Nigeria.

出版信息

iScience. 2023 Apr 28;26(5):106772. doi: 10.1016/j.isci.2023.106772. eCollection 2023 May 19.

DOI:10.1016/j.isci.2023.106772

PMID:37250304

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10214733/

Abstract

species have great application prospects in textile and phytoremediation of saline soil, are rich in flavonoids, and possess medicinal significance. Here, we report the draft genomes of and , and elucidate their evolutionary relationship. The high synteny and collinearity between the two suggested that they have experienced the same WGD event. Comparative analysis revealed that flavone 3-hydroxylase () and differentially evolved flavonoid 3--glucosyl transferase () genes are critical for determining natural variation in flavonoid biosynthesis between the species. Overexpression of enhanced the total flavonoid content and promoted the antioxidant capacity of transformed plants compared to the wild-type. and explained the diversification of flavonoids or their derivatives. These data provide biochemical insight and knowledge on the genetic regulation of flavonoid biosynthesis, supporting the adoption of these genes in breeding programs aimed at the multipurpose utilization of the plants.

摘要

该物种在纺织品和盐碱地植物修复方面具有巨大的应用前景，富含黄酮类化合物，并具有药用价值。在此，我们报告了[物种名称1]和[物种名称2]的基因组草图，并阐明了它们的进化关系。两者之间高度的共线性和同线性表明它们经历了相同的全基因组复制（WGD）事件。比较分析表明，黄酮3-羟化酶（F3H）和差异进化的黄酮类3-O-葡萄糖基转移酶（UFGT）基因对于确定这两个物种间黄酮类生物合成的自然变异至关重要。与野生型相比，F3H的过表达提高了转基因植物的总黄酮含量并增强了其抗氧化能力。F3H和UFGT解释了黄酮类化合物或其衍生物的多样化。这些数据为黄酮类生物合成的遗传调控提供了生化见解和知识，支持在旨在对这些植物进行多用途利用的育种计划中采用这些基因。