Suppr超能文献

基于香草醛的功能化策略构建用于治疗炎症和再生椎间盘的多功能微球。

Vanillin-based functionalization strategy to construct multifunctional microspheres for treating inflammation and regenerating intervertebral disc.

作者信息

Zhu Zhuang, Yu Qifan, Li Hanwen, Han Feng, Guo Qianping, Sun Heng, Zhao He, Tu Zhengdong, Liu Zhuang, Zhu Caihong, Li Bin

机构信息

Orthopedic Institute, Department of Orthopedic Surgery, The First Affiliated Hospital, School of Biology & Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou, Jiangsu, 215007, China.

Children's Hospital of Soochow University, Pediatric Research Institute of Soochow University, Suzhou, Jiangsu, 215123, China.

出版信息

Bioact Mater. 2023 May 23;28:167-182. doi: 10.1016/j.bioactmat.2023.05.005. eCollection 2023 Oct.

Abstract

Intervertebral disc degeneration (IVDD) is one of the main causes of low back pain. Although local delivery strategies using biomaterial carriers have shown potential for IVDD treatment, it remains challenging for intervention against multiple adverse contributors by a single delivery platform. In the present work, we propose a new functionalization strategy using vanillin, a natural molecule with anti-inflammatory and antioxidant properties, to develop multifunctional gelatin methacrylate (GelMA) microspheres for local delivery of transforming growth factor β3 (TGFβ3) toward IVDD treatment. , functionalized microspheres not only improved the release kinetics of TGFβ3 but also effectively inhibited inflammatory responses and promoted the secretion of extracellular matrix (ECM) in lipopolysaccharide-induced nucleus pulposus (NP) cells. , functionalized platform plays roles in alleviating inflammation and oxidative stress, preserving the water content of NP and disc height, and maintaining intact structure and biomechanical functions, thereby promoting the regeneration of IVD. High-throughput sequencing suggests that inhibition of the phosphatidylinositol 3-kinase (PI3K)-Akt signaling might be associated with their therapeutic effects. In summary, the vanillin-based functionalization strategy provides a novel and simple way for packaging multiple functions into a single delivery platform and holds promise for tissue regeneration beyond the IVD.

摘要

椎间盘退变(IVDD)是腰痛的主要原因之一。尽管使用生物材料载体的局部递送策略已显示出治疗IVDD的潜力,但通过单一递送平台干预多种不利因素仍然具有挑战性。在本研究中,我们提出了一种新的功能化策略,使用具有抗炎和抗氧化特性的天然分子香草醛,来开发多功能甲基丙烯酸明胶(GelMA)微球,用于向IVDD治疗局部递送转化生长因子β3(TGFβ3)。功能化微球不仅改善了TGFβ3的释放动力学,还有效抑制了炎症反应,并促进了脂多糖诱导的髓核(NP)细胞中细胞外基质(ECM)的分泌。功能化平台在减轻炎症和氧化应激、保持NP的含水量和椎间盘高度、维持完整结构和生物力学功能方面发挥作用,从而促进椎间盘的再生。高通量测序表明,抑制磷脂酰肌醇3激酶(PI3K)-Akt信号传导可能与其治疗效果有关。总之,基于香草醛的功能化策略为将多种功能包装到单一递送平台提供了一种新颖且简单的方法,并有望用于椎间盘以外的组织再生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a88/10225820/08fdbf85a0d0/ga1.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验