Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.
Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP, Sao Paulo, SP, Brazil.
Eur Arch Otorhinolaryngol. 2023 Sep;280(9):4261-4269. doi: 10.1007/s00405-023-08041-6. Epub 2023 May 31.
The aim of this study was to evaluate whether sleep deprivation can induce degenerative changes in rat sublingual glands.
For this purpose, a total of 24 males were distributed into three groups: control (n = 8), in which the animals were not subjected to any procedure; sleep deprivation (n = 8) in which the animals were submitted to sleep deprivation for 96 h; recovery (n = 8), in which the animals were subjected to paradoxical sleep deprivation for 96 consecutive hours followed by 96 h without intervention. Morphological changes in sublingual glands as well as the immunoexpressions of some proteins, such as Ki-67, p16, cleaved caspase-3 and BCL-2 were investigated in this setting.
The results showed that paradoxical sleep deprivation induced tissue degeneration as a result of the presence of pyknosis, vacuoles and areas of salivary retention, in the experimental groups. Expression of cleaved caspase 3 and BCL-2 were increased in both sleep deprivation and recovery groups. The analysis of Ki-67 showed an increase in expression only in the recovery group, associated with a decrease in p16 levels.
Sleep deprivation can induce a degenerative process in the parenchyma of sublingual gland by means of dysregulation of apoptosis associated with proliferative activity.
本研究旨在评估睡眠剥夺是否会引起大鼠舌下腺的退行性变化。
为此，共将 24 只雄性大鼠分为三组：对照组（n = 8，动物未进行任何处理）；睡眠剥夺组（n = 8，动物接受 96 小时的睡眠剥夺）；恢复组（n = 8，动物接受连续 96 小时的异相睡眠剥夺后，无干预 96 小时）。在这种情况下，研究了舌下腺的形态变化以及某些蛋白质（如 Ki-67、p16、cleaved caspase-3 和 BCL-2）的免疫表达。
结果表明，实验各组中，异相睡眠剥夺导致组织退化，表现为核固缩、空泡和唾液潴留区。cleaved caspase 3 和 BCL-2 的表达在睡眠剥夺和恢复组均增加。Ki-67 的分析显示仅在恢复组表达增加，同时 p16 水平降低。
睡眠剥夺可通过与增殖活性相关的细胞凋亡失调，引起舌下腺实质的退行性过程。
