Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil.
Med Princ Pract. 2021;30(3):253-261. doi: 10.1159/000515307. Epub 2021 Feb 18.
The aim of the study was to study the Janus kinase/tyrosine kinase-activated transduction factor (JAK/STAT) signaling pathway and myogenesis on the masseter muscle after sleep deprivation and to investigate the role of stress in this scenario.
A total of 18 male Wistar rats were divided into the following groups: control (n = 6): animals were not submitted to any procedures, and paradoxical sleep deprivation and vehicle (PSD + V; n = 6): animals were subjected to PSD for 96 h and (PSD + MET; n = 6): animals were subjected to PSD for 96 h with administration of metyrapone. Paradoxical sleep deprivation was performed by the modified multiple platforms method. Histopathological analysis, histomorphometry, and immunohistochemistry were performed.
The results showed the presence of inflammatory infiltrate in the PSD + V and PSD + MET groups and atrophy. Histomorphometry showed that the cellular profile area decreased, while cellular density increased in both experimental groups. Expression of p-STAT 3, MyoD, and MyoG increased in the PSD + V group, while the PSD + MET group showed increased expression of IL-6 and p-STAT 3.
Our results suggest that sleep deprivation induces an inflammatory response and atrophy in the masseter muscle of rats.
本研究旨在研究睡眠剥夺后咀嚼肌中的 Janus 激酶/酪氨酸激酶激活转录因子（JAK/STAT）信号通路和肌生成，并探讨应激在此过程中的作用。
将 18 只雄性 Wistar 大鼠分为以下几组：对照组（n = 6）：动物未进行任何处理；睡眠剥夺和载体组（PSD + V；n = 6）：动物进行 96 小时的睡眠剥夺，并给予美替拉酮；睡眠剥夺和 MET 组（PSD + MET；n = 6）：动物进行 96 小时的睡眠剥夺，并给予美替拉酮。采用改良多平台法进行异相睡眠剥夺。进行组织病理学分析、组织形态计量学和免疫组织化学检测。
结果显示，在 PSD + V 和 PSD + MET 组中存在炎症浸润和萎缩。组织形态计量学显示，两个实验组的细胞形态面积减小，而细胞密度增加。PSD + V 组中 p-STAT3、MyoD 和 MyoG 的表达增加，而 PSD + MET 组中 IL-6 和 p-STAT3 的表达增加。
我们的结果表明，睡眠剥夺可诱导大鼠咀嚼肌发生炎症反应和萎缩。
