计算性表位嫁接。

Computational Grafting of Epitopes.

机构信息

School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi, India.

Indian Council of Medical Research, New Delhi, India.

出版信息

Methods Mol Biol. 2023;2673:111-122. doi: 10.1007/978-1-0716-3239-0_7.

DOI:10.1007/978-1-0716-3239-0_7

PMID:37258909

Abstract

Epitopes are the cornerstones for the development of rational vaccine design strategies. Conventionally, epitopes are used by chemical conjugation with the carrier protein. This chapter describes our computational epitope grafting methodology to identify the preferential grafting site in a carrier protein/scaffold. We have used the mota epitope as an example, as it was already experimentally validated by an independent group. In this chapter, we have provided sufficient details to enable the wet experimentalist to employ this computational methodology in their research objective. Scripts/programs are extensively described in this chapter and freely accessible through the provided link.

摘要

表位是理性疫苗设计策略的基石。传统上，表位是通过与载体蛋白的化学偶联来使用的。本章描述了我们的计算表位嫁接方法，以确定载体蛋白/支架中的优先嫁接位点。我们使用 mota 表位作为一个例子，因为它已经被一个独立的小组通过实验验证。在本章中，我们提供了足够的细节，使湿实验者能够在他们的研究目标中使用这种计算方法。脚本/程序在本章中有详细的描述，并可通过提供的链接免费访问。