Laboratorio de Diseño y Desarrollo de Nuevos Fármacos e Innovación Biotécnológica (Laboratory for the Design and Development of New Drugs and Biotechnological Innovation), Escuela Superior de Medicina, Instituto Politécnico Nacional, México City, Mexico.
Laboratorio de medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional, México City, Mexico.
Methods Mol Biol. 2022;2412:471-479. doi: 10.1007/978-1-0716-1892-9_26.
Epitope-based vaccines is one of the most recent methodologies applied in bioinformatics studies. This strategy consists of identifying regions of the protein (peptides or epitopes) which show antigen properties capable of stimulating the immune system against proteins from virus, bacteria, fungi, etc. This chapter describes a general procedure to identify epitopes to be used as epitope vaccine using bioinformatics methods including primary protein sequence analyses, epitope predictor, docking, and molecular dynamics simulations for the selection of T- and B-cell epitopes.
基于表位的疫苗是生物信息学研究中应用的最新方法之一。该策略包括鉴定蛋白质（肽或表位）中的区域，这些区域显示出抗原特性，能够刺激免疫系统对抗来自病毒、细菌、真菌等的蛋白质。本章描述了一种使用生物信息学方法识别用作表位疫苗的表位的一般程序，包括对初级蛋白质序列的分析、表位预测、对接和分子动力学模拟，以选择 T 细胞和 B 细胞表位。
