Department of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.
Medical School of Ophthalmology & Optometry, North Sichuan Medical College, Nanchong, Sichuan, China.
Invest Ophthalmol Vis Sci. 2023 Jun 1;64(7):1. doi: 10.1167/iovs.64.7.1.
To identify the expression of the mechanosensitive ion channel Piezo1 in the retina of guinea pigs with form deprivation myopia (FDM) and to investigate mechanisms by which Piezo1 channels might regulate myopia.
Sixty 3-week-old guinea pigs were divided into four groups randomly: normal control, FDM, FDM + vehicle control (DMSO), and FDM + Piezo1 inhibitor (GsMTx4). Measurements of spherical equivalent (SE) and axial length (AL) of the guinea pig were taken using retinoscopy and A-scan ultrasound examination, respectively. Location of Piezo1 protein was determined using immunohistochemistry. The histological structure and thickness changes of the guinea pig retina were observed by hematoxylin and eosin. Expression of Piezo1 in the retina was detected using quantitative RT-PCR and Western blot. Reactive oxygen species (ROS) levels in the retina were measured using flow cytometry.
After 4 weeks of form deprivation, the FDM group exhibited a significantly increased myopic degree and axial length compared with the normal control group (all P < 0.001), and had higher expression levels of Piezo1 and ROS than the normal control group (P < 0.001 and P = 0.002, respectively). Piezo1 protein expression was down-regulated in guinea pigs given GsMTx4 compared with the DMSO group (P = 0.037). Additionally, the GsMTx4 group showed lower myopic degree (P < 0.001) and lower ROS levels (P = 0.019) compared with the DMSO group.
The Piezo1 channel may be activated in the retinas of FDM guinea pigs and be involved in the development of myopia by regulating intraocular ROS levels.
鉴定机械敏感离子通道 Piezo1 在形觉剥夺性近视（FDM）豚鼠视网膜中的表达，并研究 Piezo1 通道可能调节近视的机制。
将 60 只 3 周龄豚鼠随机分为 4 组：正常对照组、FDM 组、FDM+载体对照组（DMSO）和 FDM+Piezo1 抑制剂（GsMTx4）组。使用视网膜检影法和 A 型超声扫描分别测量豚鼠的等效球镜（SE）和眼轴（AL）。使用免疫组织化学法确定 Piezo1 蛋白的位置。通过苏木精和伊红染色观察豚鼠视网膜的组织学结构和厚度变化。使用实时定量 RT-PCR 和 Western blot 检测视网膜中 Piezo1 的表达。使用流式细胞术测量视网膜中的活性氧（ROS）水平。
在 4 周的形觉剥夺后，FDM 组与正常对照组相比，近视程度和眼轴显著增加（均 P < 0.001），且 Piezo1 和 ROS 的表达水平均高于正常对照组（均 P < 0.001 和 P = 0.002）。与 DMSO 组相比，给予 GsMTx4 的豚鼠 Piezo1 蛋白表达下调（P = 0.037）。此外，与 DMSO 组相比，GsMTx4 组的近视程度较低（P < 0.001），ROS 水平较低（P = 0.019）。
Piezo1 通道可能在 FDM 豚鼠的视网膜中被激活，并通过调节眼内 ROS 水平参与近视的发生发展。
Zotero 插件
