Wu Yajun, Feng Yuliang, Yang Jiasong, Fan Hua, Yu Zitong, Xie Xiaolin, Dai Yumeng, Huang Xin, Li Wensheng
Aier School of Ophthalmology, Central South University, Changsha, Hunan, China.
Department of Ophthalmology, Shanghai Aier Ophthalmology Hospital, Shanghai, China.
Front Cell Dev Biol. 2023 Mar 17;11:1160897. doi: 10.3389/fcell.2023.1160897. eCollection 2023.
Myopia is a common chronic eye disease, this study is to investigate the effects of exogenous retinoic acid (RA) on intraocular parameters, especially choroidal thickness (CT) and retinal thickness (RT), in guinea pigs with form deprivation myopia (FDM). A total of 80 male guinea pigs were divided randomly into 4 groups: Control, FDM, FDM + RA, and FDM + Citral groups. The FDM + RA group was given 24 mg/kg RA dissolved in 0.4 mL peanut oil; the FDM + Citral group was given citral 445 mg/kg dissolved in 0.4 mL peanut oil; The other two groups were given 0.4 mL peanut oil. After 4 weeks, the refractive error (RE), axial length (AL), and intraocular pressure (IOP) of all guinea pigs were measured, and the parameters of RT and CT were obtained using enhanced depth imaging optical coherence tomography (EDI-OCT). After 4 weeks, both the RE and AL in the FDM and FDM + RA groups were increased, and the RT and CT in both groups were smaller than those in the Control group ( < 0.05). Only the IOP of the right eye in the FDM + RA group increased significantly ( < 0.05). The RT of the right eye of the 4 groups was compared: Control group > FDM + Citral group > FDM group > FDM + RA group. Compared with the RT of the left eye and the right eye among the 4 groups, the RT of the right eye in the FDM and FDM + RA groups was significantly less than that in the left eye ( < 0.05). Moreover, the CT of the right eye in the Control group was greater than that in the other three groups ( < 0.0001). There was no significant difference in the CT among the FDM, FDM + RA, and FDM + Citral groups ( > 0.05). In contrast to the RT results, the CT results of the left and right eyes in the FDM + Citral group showed statistically significant differences ( < 0.05). RA participates in the progression of FDM as a regulatory factor. Exogenous RA can increase the RE, AL, and IOP of FDM guinea pigs, and might aggravate the retinal thinning of FDM guinea pigs. Citral can inhibit these changes, but RA might not affect the thickness of the choroid.
近视是一种常见的慢性眼病，本研究旨在探讨外源性视黄酸（RA）对形觉剥夺性近视（FDM）豚鼠眼内参数的影响，尤其是脉络膜厚度（CT）和视网膜厚度（RT）。总共80只雄性豚鼠被随机分为4组：对照组、FDM组、FDM + RA组和FDM + 柠檬醛组。FDM + RA组给予溶解于0.4 mL花生油中的24 mg/kg RA；FDM + 柠檬醛组给予溶解于0.4 mL花生油中的445 mg/kg柠檬醛；另外两组给予0.4 mL花生油。4周后，测量所有豚鼠的屈光不正（RE）、眼轴长度（AL）和眼压（IOP），并使用增强深度成像光学相干断层扫描（EDI - OCT）获得RT和CT参数。4周后，FDM组和FDM + RA组的RE和AL均增加，且两组的RT和CT均小于对照组（P < 0.05）。仅FDM + RA组右眼的IOP显著升高（P < 0.05）。比较4组右眼的RT：对照组 > FDM + 柠檬醛组 > FDM组 > FDM + RA组。比较4组左右眼的RT，FDM组和FDM + RA组右眼的RT显著小于左眼（P < 0.05）。此外，对照组右眼的CT大于其他三组（P < 0.0001）。FDM组、FDM + RA组和FDM + 柠檬醛组之间的CT无显著差异（P > 0.05）。与RT结果相反，FDM + 柠檬醛组左右眼的CT结果显示出统计学上的显著差异（P < 0.05）。RA作为一种调节因子参与FDM的进展。外源性RA可增加FDM豚鼠的RE、AL和IOP，并可能加重FDM豚鼠的视网膜变薄。柠檬醛可抑制这些变化，但RA可能不影响脉络膜厚度。
