Research Center of Basic Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, People's Republic of China.
Nanotechnology. 2023 Jun 23;34(36). doi: 10.1088/1361-6528/acdaa1.
Oxidative stress caused by excessive reactive oxygen species (ROS) leads to the dysfunction of white adipocytes and white fat, and also promotes triglyceride storage by inhibiting the respiration of adipocytes directly. Nanozymes, as a new generation of artificial enzymes, have exhibited attractive potential in scavenging ROS and treatment of ROS-related diseases. Herein, aptamer-modified atomically precise gold Aunanoclusters (Apt-AuNCs), are employed as targeted nanozymes to scavenge ROS in white adipocytes. Our results show that Apt-AuNCs have high targeting capability toward white adipocytes with low cytotoxicity. Furthermore, Apt-AuNCs show high superoxide dismutase (SOD)-like and catalase (CAT)-like activity in a concentration-dependent manner, and also good thermal and pH stability compared with natural SOD and CAT. Finally, the efficiency of ROS scavenging by Apt-AuNCs in white adipocytes is evaluated. This work demonstrates that Apt-AuNCs, as targeted nanozymes, are efficient in scavenging ROS in white adipocytes, exhibiting promising potential for the treatment of obesity and related diseases.
由过量活性氧 (ROS) 引起的氧化应激导致白色脂肪细胞和白色脂肪的功能障碍，并通过直接抑制脂肪细胞的呼吸促进甘油三酯的储存。纳米酶作为新一代人工酶，在清除 ROS 和治疗 ROS 相关疾病方面表现出了诱人的潜力。本文中，适配体修饰的原子精确金纳米团簇（Apt-AuNCs）被用作靶向纳米酶来清除白色脂肪细胞中的 ROS。我们的结果表明，Apt-AuNCs 对白色脂肪细胞具有高靶向性和低细胞毒性。此外，Apt-AuNCs 表现出高的超氧化物歧化酶 (SOD)-样和过氧化氢酶 (CAT)-样活性，并且与天然 SOD 和 CAT 相比具有良好的热稳定性和 pH 稳定性。最后，评估了 Apt-AuNCs 在白色脂肪细胞中清除 ROS 的效率。这项工作表明，Apt-AuNCs 作为靶向纳米酶，能够有效地清除白色脂肪细胞中的 ROS，为肥胖症和相关疾病的治疗提供了有前景的应用。
