State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
Int J Biol Macromol. 2023 Jul 1;242(Pt 4):125162. doi: 10.1016/j.ijbiomac.2023.125162. Epub 2023 May 30.
Salt stress is an abiotic stress factor that limits high yields, and thus identifying salt tolerance genes is very important for improving the tolerance of salt in wheat. In this study we identified 274 TaRING-H2 family members and analyzed their gene positions, gene structures, conserved structural domains, promoter cis-acting elements and covariance relationships. And we investigated TaRING-H2-120 (TaSDIR1) in salt stress. Transgenic lines exhibited higher salt tolerance in the germination and seedling stages. Compared with the wild type, overexpression of TaSDIR1 upregulated the expression of genes encoding enzymes related to the control of reactive oxygen species (ROS), thereby reducing the accumulation of ROS, as well as increased the expression of ion transport-related genes to limit the inward flow of Na in vivo and maintain a higher K/Na ratio. The expression levels of these genes were opposite in lines where TaSDIR1 was silenced by BSMV-VIGS, and the silenced wheat exhibited higher salt sensitivity. Arabidopsis mutants and heterologous TaSDIR1 overexpressing lines had similar salt stress tolerance phenotypes. We also demonstrated that TaSDIR1 interacted with TaSDIR1P2 in vivo and in vitro. A sequence of 80-100 amino acids in TaSDIR1P2 encoded a coiled coil domain that was important for the activity of E3 ubiquitin ligase, and it was also the core region for the interaction between TaSDIR1 and TaSDIR1P2. Overall, our results suggest that TaSDIR1 positively regulates salt stress tolerance in wheat.
盐胁迫是限制高产的非生物胁迫因素，因此鉴定耐盐基因对于提高小麦的耐盐性非常重要。本研究鉴定了 274 个 TaRING-H2 家族成员，并分析了它们的基因位置、基因结构、保守结构域、启动子顺式作用元件和共线性关系。我们还研究了 TaRING-H2-120（TaSDIR1）在盐胁迫下的功能。转基因株系在萌发和幼苗期表现出更高的耐盐性。与野生型相比，过表达 TaSDIR1 上调了与活性氧（ROS）调控相关的酶编码基因的表达，从而减少 ROS 的积累，同时增加了离子转运相关基因的表达，以限制体内 Na 的内流并维持更高的 K/Na 比。沉默 TaSDIR1 的 BSMV-VIGS 株系中这些基因的表达水平相反，沉默的小麦表现出更高的盐敏感性。拟南芥突变体和异源 TaSDIR1 过表达株系具有相似的耐盐胁迫表型。我们还证明了 TaSDIR1 在体内和体外与 TaSDIR1P2 相互作用。TaSDIR1P2 中 80-100 个氨基酸的序列编码一个卷曲螺旋结构域，该结构域对于 E3 泛素连接酶的活性很重要，也是 TaSDIR1 和 TaSDIR1P2 相互作用的核心区域。总之，我们的结果表明 TaSDIR1 正向调控小麦的耐盐性。
