Simmon V F
J Natl Cancer Inst. 1979 Apr;62(4):893-9.
The mutagenic activity of 101 chemicals was studied with the use of the Salmonella typhimurium-microsome system described by Ames. The tester strains were TA1535, TA1536, TA1537, TA1538, TA98, and TA100. Assays were conducted in the presence and absence of a metabolic activation system prepared from the livers of randomly bred Sprague-Dawley rats that had been pretreated with Aroclor 1254. The test chemicals were incorporated into the agar with bacteria and the metabolic activation system. Mutagens were defined as chemicals that induced a reproducible dose-related increase in the number of histidine-independent revertants. With the use of these procedures, 65% of the organic carcinogens and 25% of the noncarcinogens were found to be mutagenic.
利用艾姆斯描述的鼠伤寒沙门氏菌-微粒体系统，研究了101种化学物质的致突变活性。测试菌株为TA1535、TA1536、TA1537、TA1538、TA98和TA100。在有和没有由用多氯联苯1254预处理过的随机繁殖的斯普拉格-道利大鼠肝脏制备的代谢活化系统的情况下进行测定。将测试化学物质与细菌及代谢活化系统一起掺入琼脂中。诱变剂定义为能引起组氨酸非依赖型回复突变体数量出现可重复的剂量相关增加的化学物质。采用这些程序，发现65%的有机致癌物和25%的非致癌物具有致突变性。
