Khaled Jamal M, Alharbi Naiyf S, Al-Huwaymil Rayan M, Kadaikunnan Shine, Alobaidi Ahmed S, Alfalih Abdullah M
Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2023 Jun;30(6):103680. doi: 10.1016/j.sjbs.2023.103680. Epub 2023 May 5.
The stability of drugs in biological evidence during collection and storage is of particular concern in forensic investigations. Microbes in the samples and other elements are an essential component of these investigations. In the current study, the HPLC method was used to examine the stability of (1R, 2S)-(-)-Ephedrine hydrochloride in plasma and urine samples inoculated with after storage at 37 °C for 48 h and -20 °C for six months. In the stability experiment, MIC of (1R, 2S)-(-)-Ephedrine hydrochloride was applied according to MIC and MFC that were determined in this work. This drug had MIC and MFC of 50 and 100 ppm, respectively. In HPLC analysis, the standard (1R, 2S)-(-)-Ephedrine hydrochloride had a retention time of 1.63 and was used to identify this drug in samples that had or had not been exposed to . The findings demonstrated that within 48 h at 37 °C, had an impact on the drug concentration (10% and more than 15%, respectively, were lost in plasma and urine samples inoculated with ). In plasma samples, the drug content remained stable at -20 °C for three months, although it degraded in urine samples after one month. In plasma and urine samples, the concentration reduction had surpassed 70% and 50% by the sixth month, respectively. The results of this investigation show that can change the stability of (1R, 2S)-(-)-Ephedrine hydrochloride in plasma and urine samples that contain MIC of Ephedrine hydrochloride.
在法医调查中，生物证据中药物在采集和储存过程中的稳定性备受关注。样本中的微生物和其他因素是这些调查的重要组成部分。在本研究中，采用高效液相色谱法（HPLC）检测了（1R，2S）-（-）-盐酸麻黄碱在接种了[具体物质未给出]的血浆和尿液样本中，于37°C储存48小时以及-20°C储存六个月后的稳定性。在稳定性实验中，根据本研究确定的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）应用（1R，2S）-（-）-盐酸麻黄碱的MIC。该药物的MIC和MFC分别为50 ppm和100 ppm。在HPLC分析中，标准（1R，2S）-（-）-盐酸麻黄碱的保留时间为1.63，用于鉴定已暴露或未暴露于[具体物质未给出]的样本中的该药物。研究结果表明，在37°C下48小时内，[具体物质未给出]对药物浓度有影响（接种了[具体物质未给出]的血浆和尿液样本中分别损失了10%和超过15%）。在血浆样本中，药物含量在-20°C下三个月保持稳定，尽管在尿液样本中一个月后就开始降解。到第六个月时，血浆和尿液样本中的浓度降低分别超过了70%和50%。本调查结果表明，[具体物质未给出]可改变含有盐酸麻黄碱MIC的血浆和尿液样本中（1R，2S）-（-）-盐酸麻黄碱的稳定性。
