El-Baz Ahmed M, Mosbah Rasha A, Goda Reham M, Mansour Basem, Sultana Taranum, Dahms Tanya E S, El-Ganiny Amira M
Microbiology and Biotechnology Department, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa City, Mansoura, 11152 Dakhaliya, Egypt.
Infection Control Unit, Zagazig University Hospitals, 44519 Zagazig, Egypt.
Antibiotics (Basel). 2021 Jan 15;10(1):81. doi: 10.3390/antibiotics10010081.
is the causative agent of fatal systemic candidiasis. Due to limitations of antifungals, new drugs are needed. The anti-virulence effect of plant essential oils (EOs) was evaluated against clinical isolates including cinnamon, clove, jasmine and rosemary oils. Biofilm, phospholipase and hemolysin were assessed phenotypically. EOs were evaluated for their anti-virulence activity using phenotypic methods as well as scanning electron microscopy (SEM) and atomic force microscopy (AFM). Among the isolates, biofilm, phospholipase and hemolysins were detected in 40.4, 86.5 and 78.8% of isolates, respectively. Jasmine oil showed the highest anti-biofilm activity followed by cinnamon, clove and rosemary oils. SEM and AFM analysis showed reduced adherence and roughness in the presence of EOs. For phospholipase, rosemary oil was the most inhibitory, followed by jasmine, cinnamon and clove oils, and for hemolysins, cinnamon had the highest inhibition followed by jasmine, rosemary and clove oils. A molecular docking study revealed major EO constituents as promising inhibitors of the Als3 adhesive protein, with the highest binding for eugenol, followed by 1,8-cineole, 2-phenylthiolane and cinnamaldehyde. In conclusion, EOs have a promising inhibitory impact on Candida biofilm, phospholipase and hemolysin production, hence EOs could be used as potential antifungals that impact virulence factors.
是致命性系统性念珠菌病的病原体。由于抗真菌药物存在局限性，需要研发新药。对包括肉桂油、丁香油、茉莉油和迷迭香油在内的植物精油（EOs）针对临床分离株的抗毒力作用进行了评估。对生物膜、磷脂酶和溶血素进行了表型评估。使用表型方法以及扫描电子显微镜（SEM）和原子力显微镜（AFM）对EOs的抗毒力活性进行了评估。在所检测的分离株中，分别有40.4%、86.5%和78.8%的分离株检测到生物膜、磷脂酶和溶血素。茉莉油显示出最高的抗生物膜活性，其次是肉桂油、丁香油和迷迭香油。SEM和AFM分析表明，在EOs存在的情况下，念珠菌的黏附性和粗糙度降低。对于磷脂酶，迷迭香油的抑制作用最强，其次是茉莉油、肉桂油和丁香油；对于溶血素，肉桂油的抑制作用最强，其次是茉莉油、迷迭香油和丁香油。分子对接研究表明，EOs的主要成分是Als3黏附蛋白的有前景的抑制剂，其中丁香酚的结合能力最强，其次是1,8-桉叶素、2-苯基硫杂环戊烷和肉桂醛。总之，EOs对念珠菌生物膜、磷脂酶和溶血素的产生具有有前景的抑制作用，因此EOs可作为影响毒力因子的潜在抗真菌药物。
