Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori, Japan.
Centre for Research on Green Sustainable Chemistry, Tottori University, Tottori, Japan.
PLoS One. 2023 Jun 2;18(6):e0286421. doi: 10.1371/journal.pone.0286421. eCollection 2023.
Studies on how exogenous molecules modulate properties of plant microtubules, such as their stability, structure, and dynamics, are important for understanding and modulating microtubule functions in plants. We have developed a Tau-derived peptide (TP) that binds to microtubules and modulates their properties by binding of TP-conjugated molecules in vitro. However, there was no investigation of TPs on microtubules in planta. Here, we generated transgenic Arabidopsis thaliana plants stably expressing TP-fused superfolder GFP (sfGFP-TP) and explored the binding properties and effects of sfGFP-TP on plant microtubules. Our results indicate that the expressed sfGFP-TP binds to the plant microtubules without inhibiting plant growth. A transgenic line strongly expressing sfGFP-TP produced thick fibrous structures that were stable under conditions where microtubules normally depolymerize. This study generates a new tool for analyzing and modulating plant microtubules.
研究外源分子如何调节植物微管的性质，如稳定性、结构和动态，对于理解和调节植物微管的功能非常重要。我们开发了一种来源于微管蛋白的肽（TP），它通过与体外结合的 TP 缀合分子结合来调节微管的性质。然而，在植物体内还没有对 TPs 与微管相互作用的研究。在这里，我们生成了稳定表达 TP 融合超折叠 GFP（sfGFP-TP）的拟南芥转基因植物，并探索了 sfGFP-TP 对植物微管的结合特性和影响。我们的结果表明，表达的 sfGFP-TP 与植物微管结合，而不抑制植物生长。强烈表达 sfGFP-TP 的转基因系产生了厚的纤维状结构，在微管通常解聚的条件下仍然稳定。这项研究为分析和调节植物微管提供了一种新工具。
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