Guangdong Institute of Intelligence Science and Technology, Hengqin 519031, Zhuhai, Guangdong, China; Research Unit of Pain Medicine, Chinese Academy of Medical Sciences, Hengqin, Zhuhai, China.
Guangdong Institute of Intelligence Science and Technology, Hengqin 519031, Zhuhai, Guangdong, China; Research Unit of Pain Medicine, Chinese Academy of Medical Sciences, Hengqin, Zhuhai, China; Xuhui Central Hospital, Shanghai, 200031, China.
Trends Neurosci. 2023 Aug;46(8):654-666. doi: 10.1016/j.tins.2023.05.005. Epub 2023 May 31.
Single-cell RNA sequencing (scRNA-seq) has allowed profiling cell types of the dorsal root ganglia (DRG) and their transcriptional states in physiology and chronic pain. However, the evaluation criteria used in previous studies to classify DRG neurons varied, which presents difficulties in determining the various types of DRG neurons. In this review, we aim to integrate findings from previous transcriptomic studies of the DRG. We first briefly introduce the history of DRG-neuron cell-type profiling, and discuss the advantages and disadvantages of different scRNA-seq methods. We then examine the classification of DRG neurons based on single-cell profiling under physiological and pathological conditions. Finally, we propose further studies on the somatosensory system at the molecular, cellular, and neural network levels.
单细胞 RNA 测序 (scRNA-seq) 已经能够对背根神经节 (DRG) 的细胞类型及其在生理和慢性疼痛中的转录状态进行分析。然而，以前研究中用于分类 DRG 神经元的评估标准各不相同，这给确定各种类型的 DRG 神经元带来了困难。在这篇综述中，我们旨在整合 DRG 转录组研究的结果。我们首先简要介绍 DRG 神经元细胞类型分析的历史，并讨论不同 scRNA-seq 方法的优缺点。然后，我们检查在生理和病理条件下基于单细胞分析对 DRG 神经元的分类。最后，我们提出在分子、细胞和神经网络水平上对感觉系统进行进一步研究的建议。
