Institute of Botany, Faculty of Biology, Jagiellonian University, Gronostajowa 3, 30-387, Kraków, Poland.
Institute of Botany, Czech Academy of Sciences, Zámek 1, 252 43, Průhonice, Czech Republic.
Sci Rep. 2023 Jun 3;13(1):9030. doi: 10.1038/s41598-023-36253-7.
Understanding the genetic variability of hybrids provides information on their current and future evolutionary role. In this paper, we focus on the interspecific hybrid Ranunculus circinatus × R. fluitans that forms spontaneously within the group Ranuculus L. sect. Batrachium DC. (Ranunculaceae Juss.). Genome-wide DNA fingerprinting using amplified fragment length polymorphisms (AFLP) was employed to determine the genetic variation among 36 riverine populations of the hybrid and their parental species. The results demonstrate a strong genetic structure of R. circinatus × R. fluitans within Poland (Central Europe), which is attributed to independent hybridization events, sterility of hybrid individuals, vegetative propagation, and isolation through geographical distance within populations. The hybrid R. circinatus × R. fluitans is a sterile triploid, but, as we have shown in this study, it may participate in subsequent hybridization events, resulting in a ploidy change that can lead to spontaneous fertility recovery. The ability to produce unreduced female gametes of the hybrid R. circinatus × R. fluitans and the parental species R. fluitans is an important evolutionary mechanism in Ranunculus sect. Batrachium that could give rise to new taxa.
了解杂种的遗传变异性可以提供有关其当前和未来进化作用的信息。在本文中，我们专注于 Ranunculus L.sect. Batrachium DC.（毛茛科）内自然形成的种间杂种Ranunculus circinatus × R. fluitans。利用扩增片段长度多态性（AFLP）进行全基因组 DNA 指纹分析，以确定杂种及其亲本种的 36 个河流种群之间的遗传变异。结果表明，波兰（中欧）的 R. circinatus × R. fluitans 具有很强的遗传结构，这归因于独立的杂交事件、杂种个体的不育性、营养繁殖以及种群内地理距离的隔离。杂种 R. circinatus × R. fluitans 是一种不育的三倍体，但正如我们在这项研究中所示，它可能参与随后的杂交事件，导致染色体数目的变化，从而导致自发的育性恢复。杂种 R. circinatus × R. fluitans 和亲本种 R. fluitans 产生未减数的雌性配子的能力是 Ranunculus sect. Batrachium 中的一个重要进化机制，可能会产生新的分类群。
