• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脊髓损伤中具有临床和生物学价值的细胞焦亡相关基因特征的综合分析

Comprehensive analysis of a pyroptosis-related gene signature of clinical and biological values in spinal cord injury.

作者信息

Zhang Pingping, Zhang Jianping, Kou Wenjuan, Gu Guangjin, Zhang Yaning, Shi Weihan, Chu Pengcheng, Liang Dachuan, Sun Guangwei, Shang Jun

机构信息

Department of Orthopedics, Seventh Affiliated Hospital of Shanxi Medical University, Linfen People's Hospital, Linfen, Shanxi, China.

Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin, China.

出版信息

Front Neurol. 2023 May 19;14:1141939. doi: 10.3389/fneur.2023.1141939. eCollection 2023.

DOI:10.3389/fneur.2023.1141939
PMID:37273699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10237016/
Abstract

BACKGROUND

Since some of the clinical examinations are not suitable for patients with severe spinal cord injury (SCI), blood biomarkers have been reported to reflect the severity of SCI. The objective of this study was to screen out the potential biomarkers associated with the diagnosis of SCI by bioinformatics analysis.

METHODS

The microarray expression profiles of SCI were obtained from the Gene Expression Omnibus (GEO) database. Core genes correlated to pyroptosis were obtained by crossing the differential genes, and module genes were obtained by WGCNA analysis and lasso regression. The immune infiltration analysis and GSEA analysis revealed the essential effect of immune cells in the progression of SCI. In addition, the accuracy of the biomarkers in diagnosing SCI was subsequently evaluated and verified using the receiver operating characteristic curve (ROC) and qRT-PCR.

RESULTS

A total of 423 DEGs were identified, among which 319 genes were upregulated and 104 genes were downregulated. Based on the WGCNA analysis, six potential biomarkers were screened out, including LIN7A, FCGR1A, FGD4, GPR27, BLOC1S1, and GALNT4. The results of ROC curves demonstrated the accurate value of biomarkers related to SCI. The immune infiltration analysis and GSEA analysis revealed the essential effect of immune cells in the progression of SCI, including macrophages, natural killer cells, and neutrophils. The qRT-PCR results verified that FGD4, FCAR1A, LIN7A, BLOC1S1, and GPR27 were significantly upregulated in SCI patients.

CONCLUSION

In this study, we identified and verified five immune pyroptosis-related hub genes by WGCNA and biological experiments. It is expected that the five identified potential biomarkers in peripheral white blood cells may provide a novel strategy for early diagnosis.

摘要

背景

由于一些临床检查不适用于严重脊髓损伤（SCI）患者，据报道血液生物标志物可反映SCI的严重程度。本研究的目的是通过生物信息学分析筛选出与SCI诊断相关的潜在生物标志物。

方法

从基因表达综合数据库（GEO）中获取SCI的微阵列表达谱。通过交叉差异基因获得与细胞焦亡相关的核心基因，并通过加权基因共表达网络分析（WGCNA）和套索回归获得模块基因。免疫浸润分析和基因集富集分析（GSEA）揭示了免疫细胞在SCI进展中的重要作用。此外，随后使用受试者工作特征曲线（ROC）和qRT-PCR评估并验证了生物标志物在诊断SCI中的准确性。

结果

共鉴定出423个差异表达基因（DEG），其中319个基因上调，104个基因下调。基于WGCNA分析，筛选出6个潜在生物标志物，包括LIN7A、FCGR1A、FGD4、GPR27、BLOC1S1和GALNT4。ROC曲线结果证明了与SCI相关的生物标志物的准确价值。免疫浸润分析和GSEA分析揭示了免疫细胞在SCI进展中的重要作用，包括巨噬细胞、自然杀伤细胞和中性粒细胞。qRT-PCR结果证实，FGD4、FCAR1A、LIN7A、BLOC1S1和GPR27在SCI患者中显著上调。

结论

在本研究中，我们通过WGCNA和生物学实验鉴定并验证了5个与免疫细胞焦亡相关的枢纽基因。预期在外周血白细胞中鉴定出的这5个潜在生物标志物可能为早期诊断提供新策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/65ca87ece979/fneur-14-1141939-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/1b455d48a499/fneur-14-1141939-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/5167743a2d71/fneur-14-1141939-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/de659b308b37/fneur-14-1141939-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/e4c4aac5496e/fneur-14-1141939-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/60cfd6ea9b3c/fneur-14-1141939-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/aa028c8dd0c5/fneur-14-1141939-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/021c0fa9647a/fneur-14-1141939-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/9a6c94f52138/fneur-14-1141939-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/eebccb0622e3/fneur-14-1141939-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/65ca87ece979/fneur-14-1141939-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/1b455d48a499/fneur-14-1141939-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/5167743a2d71/fneur-14-1141939-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/de659b308b37/fneur-14-1141939-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/e4c4aac5496e/fneur-14-1141939-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/60cfd6ea9b3c/fneur-14-1141939-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/aa028c8dd0c5/fneur-14-1141939-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/021c0fa9647a/fneur-14-1141939-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/9a6c94f52138/fneur-14-1141939-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/eebccb0622e3/fneur-14-1141939-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5862/10237016/65ca87ece979/fneur-14-1141939-g0010.jpg

相似文献

1
Comprehensive analysis of a pyroptosis-related gene signature of clinical and biological values in spinal cord injury.脊髓损伤中具有临床和生物学价值的细胞焦亡相关基因特征的综合分析
Front Neurol. 2023 May 19;14:1141939. doi: 10.3389/fneur.2023.1141939. eCollection 2023.
2
Identification of age-specific biomarkers of spinal cord injury: A bioinformatics analysis of young and aged mice models.鉴定脊髓损伤的年龄特异性生物标志物：年轻和老年小鼠模型的生物信息学分析。
Brain Behav. 2023 Dec;13(12):e3293. doi: 10.1002/brb3.3293. Epub 2023 Nov 30.
3
Identifying signature genes and their associations with immune cell infiltration in spinal cord injury.识别脊髓损伤中的特征基因及其与免疫细胞浸润的关联。
IBRO Neurosci Rep. 2024 Sep 21;17:320-328. doi: 10.1016/j.ibneur.2024.09.002. eCollection 2024 Dec.
4
Dysregulation and imbalance of innate and adaptive immunity are involved in the cardiomyopathy progression.先天性和适应性免疫的失调与失衡参与了心肌病的进展。
Front Cardiovasc Med. 2022 Sep 6;9:973279. doi: 10.3389/fcvm.2022.973279. eCollection 2022.
5
Screening and identification of potential hub genes and immune cell infiltration in the synovial tissue of rheumatoid arthritis by bioinformatic approach.通过生物信息学方法筛选和鉴定类风湿关节炎滑膜组织中的潜在枢纽基因及免疫细胞浸润
Heliyon. 2023 Jan 10;9(1):e12799. doi: 10.1016/j.heliyon.2023.e12799. eCollection 2023 Jan.
6
Identification of Hub Biomarkers and Immune-Related Pathways Participating in the Progression of Antineutrophil Cytoplasmic Antibody-Associated Glomerulonephritis.鉴定参与抗中性粒细胞胞浆抗体相关性肾小球肾炎进展的枢纽生物标志物和免疫相关途径。
Front Immunol. 2022 Jan 5;12:809325. doi: 10.3389/fimmu.2021.809325. eCollection 2021.
7
Identification of immune-related hub genes in spinal cord injury.鉴定脊髓损伤中的免疫相关枢纽基因。
Eur J Med Res. 2024 Oct 4;29(1):483. doi: 10.1186/s40001-024-02075-0.
8
Identification of hub biomarkers and immune cell infiltration characteristics of polymyositis by bioinformatics analysis.基于生物信息学分析鉴定多发性肌炎的枢纽生物标志物和免疫细胞浸润特征。
Front Immunol. 2022 Sep 26;13:1002500. doi: 10.3389/fimmu.2022.1002500. eCollection 2022.
9
Weighted gene co-expression network analysis reveals that CXCL10, IRF7, MX1, RSAD2, and STAT1 are related to the chronic stage of spinal cord injury.加权基因共表达网络分析表明，CXCL10、IRF7、MX1、RSAD2和STAT1与脊髓损伤的慢性期相关。
Ann Transl Med. 2021 Aug;9(15):1248. doi: 10.21037/atm-21-3586.
10
Comprehensive landscape of immune-based classifier related to early diagnosis and macrophage M1 in spinal cord injury.免疫分类器与脊髓损伤早期诊断及巨噬细胞 M1 相关的综合全景图。
Aging (Albany NY). 2023 Feb 23;15(4):1158-1176. doi: 10.18632/aging.204548.

引用本文的文献

1
Identification and validation of glucocorticoid receptor and programmed cell death-related genes in spinal cord injury using machine learning.利用机器学习鉴定和验证脊髓损伤中糖皮质激素受体和程序性细胞死亡相关基因
Sci Rep. 2025 Jul 7;15(1):24202. doi: 10.1038/s41598-025-08060-9.

本文引用的文献

1
Superconserved receptors expressed in the brain: Expression, function, motifs and evolution of an orphan receptor family.大脑中表达的超保守受体：一个孤儿受体家族的表达、功能、基序与进化
Pharmacol Ther. 2022 Dec;240:108217. doi: 10.1016/j.pharmthera.2022.108217. Epub 2022 May 26.
2
The pyroptosis-related gene signature predicts prognosis and indicates immune activity in hepatocellular carcinoma.焦亡相关基因特征可预测肝细胞癌的预后，并提示免疫活性。
Mol Med. 2022 Feb 5;28(1):16. doi: 10.1186/s10020-022-00445-0.
3
A novel pyroptosis-related signature predicts prognosis and response to treatment in breast carcinoma.
一种新的细胞焦亡相关特征可预测乳腺癌的预后和治疗反应。
Aging (Albany NY). 2022 Jan 27;14(2):989-1013. doi: 10.18632/aging.203855.
4
Identification and Validation of the Pyroptosis-Related Molecular Subtypes of Lung Adenocarcinoma by Bioinformatics and Machine Learning.通过生物信息学和机器学习鉴定及验证肺腺癌焦亡相关分子亚型
Front Cell Dev Biol. 2021 Nov 4;9:756340. doi: 10.3389/fcell.2021.756340. eCollection 2021.
5
Peripheral white blood cell responses as emerging biomarkers for patient stratification and prognosis in acute spinal cord injury.外周血白细胞反应作为急性脊髓损伤患者分层和预后的新兴生物标志物。
Curr Opin Neurol. 2021 Dec 1;34(6):796-803. doi: 10.1097/WCO.0000000000000995.
6
clusterProfiler 4.0: A universal enrichment tool for interpreting omics data.clusterProfiler 4.0：用于解释组学数据的通用富集工具。
Innovation (Camb). 2021 Jul 1;2(3):100141. doi: 10.1016/j.xinn.2021.100141. eCollection 2021 Aug 28.
7
Acute Traumatic Spinal Cord Injury.急性创伤性脊髓损伤。
Neurol Clin. 2021 May;39(2):471-488. doi: 10.1016/j.ncl.2021.02.004. Epub 2021 Mar 31.
8
Combining exome/genome sequencing with data repository analysis reveals novel gene-disease associations for a wide range of genetic disorders.结合外显子/基因组测序和数据存储库分析揭示了广泛的遗传疾病的新的基因-疾病关联。
Genet Med. 2021 Aug;23(8):1551-1568. doi: 10.1038/s41436-021-01159-0. Epub 2021 Apr 19.
9
Diagnostic blood RNA profiles for human acute spinal cord injury.用于诊断人类急性脊髓损伤的血液 RNA 图谱。
J Exp Med. 2021 Mar 1;218(3). doi: 10.1084/jem.20201795.
10
Role of pyroptosis in spinal cord injury and its therapeutic implications.焦亡在脊髓损伤中的作用及其治疗意义。
J Adv Res. 2020 Aug 18;28:97-109. doi: 10.1016/j.jare.2020.08.004. eCollection 2021 Feb.