Faye G, Dennebouy N, Kujawa C, Jacq C
Mol Gen Genet. 1979 Jan 5;168(1):101-9. doi: 10.1007/BF00267939.
The sequence organization of the yeast mit-DNA region carrying the large ribosomal RNA gene and the polar locus omega was examined. Hybridization studies using rho- deletion mutants and electron microscopy of the heteroduplexes formed between 23S rRNA and the appropriate restriction fragments, lead to the conclusion that the 23S rRNA1 gene of the omega+ strains is split by an insertion sequence of 1,000-1,100 bp. In contrast, no detactable insertion was found in the 23S rRNA gene of the omega- strains. The size and the location of the insert found in the 23S rRNA gene of the omega+ strains appear to be identical to those of the sequence delta which had previously been found to characterize the difference (at the omega locus) between the mitDNA of the wild type strains carrying the omega+ or omega- alleles (Jacq et al., 1977).
对携带大核糖体RNA基因和极性位点ω的酵母线粒体DNA区域的序列组织进行了研究。使用ρ-缺失突变体的杂交研究以及对23S rRNA与适当限制片段之间形成的异源双链体的电子显微镜观察，得出结论：ω+菌株的23S rRNA1基因被一个1000 - 1100 bp的插入序列所分割。相比之下，在ω-菌株的23S rRNA基因中未发现可检测到的插入。在ω+菌株的23S rRNA基因中发现的插入片段的大小和位置，似乎与先前发现的用于表征携带ω+或ω-等位基因的野生型菌株的线粒体DNA（在ω位点）之间差异的δ序列相同（雅克等人，1977年）。
