Dentel Alexandre, Brazhnikova Elena, Norberg Nathaniel, Jaillard Céline, Grieve Kate, Paques Michel, Sahel José A, Bertin Stéphane, Forster Valérie, Picaud Serge
Institut de la Vision, INSERM, CNRS, Sorbonne Université, Paris, France.
CHNO des Quinze-Vingts, INSERM-DGOS CIC 1423, Paris, France.
Ophthalmol Sci. 2023 Apr 20;3(4):100316. doi: 10.1016/j.xops.2023.100316. eCollection 2023 Dec.
To describe adaptive optics flood illumination ophthalmoscopy (AO-FIO) of the photoreceptor layer in normal nonhuman primates (NHPs) and in the case of a short-term induced retinal detachment (RD).
Longitudinal fundamental research study.
Four NHPs were used to image normal retinae with AO-FIO (in comparison with 4 healthy humans); 2 NHPs were used to assess the effects of RD.
The photoreceptor layer (cone mosaic metrics, including cone density, cone spacing, and cone regularity) was followed with AO-FIO imaging (rtx1, Imagine Eyes) during a surgically induced RD in 2 NHPs using a vehicle solution containing dimethyl sulfoxide, classically used as a chemical solvent. We also performed functional testing of the retina (full-field and multifocal electroretinogram [ERG]).
Correlation of cone mosaic metrics (cone density, spacing, and regularity) between normal retinae of NHPs and humans, and cone metrics, power spectrum, and ERG wave amplitudes after RD.
Imaging features were very similar in terms of cone reflectivity, cell density, regularity, and spacing values, showing strong positive correlations between NHPs and humans. After RD, AO-FIO revealed several alterations of the cone mosaic slowly recovering during the 3 months after the reattachment, which were not detected functionally by ERG.
These results demonstrate by in vivo AO-FIO imaging the transient structural changes of photoreceptors after an RD in the primate retina. They also provide an interesting illustration of the AO-FIO potential for investigating photoreceptor toxicity during preclinical studies in NHPs with a high translatability to human studies.
Proprietary or commercial disclosure may be found after the references.
描述正常非人灵长类动物（NHPs）以及短期诱导性视网膜脱离（RD）情况下光感受器层的自适应光学泛光照明检眼镜检查（AO-FIO）。
纵向基础研究。
4只NHPs用于通过AO-FIO对正常视网膜成像（与4名健康人类进行比较）；2只NHPs用于评估RD的影响。
在2只NHPs通过手术诱导RD期间，使用含有二甲基亚砜（经典用作化学溶剂）的赋形剂溶液，通过AO-FIO成像（rtx1，Imagine Eyes）跟踪光感受器层（视锥细胞镶嵌指标，包括视锥细胞密度、视锥细胞间距和视锥细胞规则性）。我们还进行了视网膜的功能测试（全视野和多焦点视网膜电图[ERG]）。
NHPs和人类正常视网膜之间视锥细胞镶嵌指标（视锥细胞密度、间距和规则性）的相关性，以及RD后视锥细胞指标、功率谱和ERG波幅。
在视锥细胞反射率、细胞密度、规则性和间距值方面，成像特征非常相似，显示NHPs和人类之间有很强的正相关性。RD后，AO-FIO显示视锥细胞镶嵌有几种改变，在视网膜复位后的3个月内缓慢恢复，而ERG在功能上未检测到这些改变。
这些结果通过体内AO-FIO成像证明了灵长类视网膜RD后光感受器的短暂结构变化。它们还提供了一个有趣的例证，说明AO-FIO在非人灵长类动物临床前研究中调查光感受器毒性方面的潜力，且对人类研究具有高度的可转化性。
专有或商业披露信息可在参考文献之后找到。
