Determination of imidacloprid in brinjal and okra fruits, decontamination and its dietary risk assessment.

Brinjal and okra are being sprayed withdifferent formulations of imidacloprid which leads to significant deposition of residues on fruits. Here in this study, we validated a method that could detect the traces of imidacloprid residues in a short run time using LC-MS/MS. LOD of 0.001 and LOQ of 0.003 μg/g for brinjal and in compliance with the MRL (0.2 mg/kg). The recovery at different spiking levels recorded 89.67 to 115.55 with an RSDr range of 3.02 and 5.22%. For okra, 0.0025 and 0.008 μg/g were observed as LOD and LOQ, respectively. Recovery was obtained between 88.69 and 93.74%. Precision in terms of repeatability (RSD) and reproducibility (RSD) was found satisfactory in both matrices. Two applications of imidacloprid 17.8% SL in both vegetables showed faster dissipation initially and persisted up to 15-25 days. Decontamination with 2% salt solution followed by boiling for 15min. removed 96.43 and 73.66% of imidacloprid residues from brinjal and okra, respectively. Risk assessment was found to be less than one (HI < 1) for brinjal and okra matrix and can be safely consumed.