大肠杆菌中林可霉素抗性突变的分析。

Analysis of lincomycin resistance mutations in Escherichia coli.

作者信息

Hummel H, Piepersberg W, Böck A

出版信息

Mol Gen Genet. 1979 Feb 1;169(3):345-7. doi: 10.1007/BF00382280.

Abstract

High level lincomycin resistant strains of Escherichia coli were isolated and screened for altered ribosomal proteins and functions. Amongst 58 strains investigated by electrophoresis one had an altered ribosomal protein S7, another one a mutated L14 and two showed altered L15 proteins. A correlation between these alterations and lincomycin resistant growth could not be demonstrated by genetic analysis for any of the mutants. In vitro, however, extracts from the two L15 mutants were less sensitive to inhibition by the drug. A gene locus (linR) responsible for the lincomycin resistance phenotype was mapped at min 30 of the Escherichia coli chromosome near tyrR; it seems to be identical to the previously described linB locus (Apirion, 1967); however, in contrast to these reports it does not seem to alter any ribosomal function.

摘要

分离并筛选出对林可霉素具有高度抗性的大肠杆菌菌株，以研究其核糖体蛋白和功能的变化。在通过电泳研究的58个菌株中，一个菌株的核糖体蛋白S7发生了改变，另一个菌株的L14发生了突变，还有两个菌株的L15蛋白发生了改变。通过遗传分析，未能证明这些变化与林可霉素抗性生长之间存在相关性。然而，在体外，两个L15突变体的提取物对该药物抑制的敏感性较低。负责林可霉素抗性表型的基因座（linR）定位在大肠杆菌染色体30分钟处靠近tyrR的位置；它似乎与先前描述的linB基因座相同（Apirion，1967）；然而，与这些报道不同的是，它似乎不会改变任何核糖体功能。