Fathizadeh Zeinab, Ghasemi Tehrani Hatave, Kazemi Mohammad, Karbasizade Vajihe
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Obstetrics and Gynecology, Isfahan University of Medical Sciences, Isfahan, Iran.
Iran J Basic Med Sci. 2023;26(6):628-634. doi: 10.22038/IJBMS.2023.66170.14545.
and species threaten reproductive health and fertility worldwide. Due to the lack of sensitive, accurate, and affordable diagnostic tools, the simultaneous contributions of these agents in infertility have been overlooked. This study aims to detect and identify and species in the genital tracts of fertile and infertile females simultaneously.
In a case-control study, cervicovaginal clinical samples were collected from patients referred to two teaching hospitals in Isfahan from July 2019 to February 2019. The initial screening was by using Real-time PCR and designed primer to evaluate the presence of and species including fertile and infertile women. The bacteria species were then detected and differentiated by using the melt curve and sequenced to confirm and identify. Finally, the standard curve was used to measure and compare the copy number of each species in each group. The isolates also were detected in clinical samples using the commercial PCR method.
The frequencies of and were (0.0, 10.0%) in the fertile group and (4.3%, 34.3%) in the infertile group, respectively. and species in the fertile group (7.1%, 5.7%) and in the infertile group (32.9%, 24.3) were determined, respectively. The comparison of the results obtained from PCR and Real-time PCR showed that the recent technique has the ability to track 101-103 copy numbers.
The present method allows differential diagnosis and quantification of and species in a short time and simultaneously.
[病原体名称]和[病原体名称]物种在全球范围内威胁生殖健康和生育能力。由于缺乏灵敏、准确且经济实惠的诊断工具，这些病原体在不孕症中的共同作用一直被忽视。本研究旨在同时检测和鉴定 fertile 和 infertile 女性生殖道中的[病原体名称]和[病原体名称]物种。
在一项病例对照研究中，于2019年7月至2019年2月从转诊至伊斯法罕两家教学医院的患者中收集宫颈阴道临床样本。初始筛查采用实时荧光定量聚合酶链反应（Real-time PCR）并使用设计的引物来评估包括 fertile 和 infertile 女性在内的[病原体名称]和[病原体名称]物种的存在情况。然后通过熔解曲线检测并区分细菌物种，并进行测序以确认和鉴定。最后，使用标准曲线测量和比较每组中每个物种的拷贝数。还使用商业PCR方法在临床样本中检测分离株。
fertile 组中[病原体名称]和[病原体名称]的频率分别为（0.0，10.0%），infertile 组中分别为（4.3%，34.3%）。fertile 组中[病原体名称]和[病原体名称]物种的频率分别为（7.1%，5.7%），infertile 组中分别为（32.9%，24.3%）。PCR和实时荧光定量聚合酶链反应（Real-time PCR）所得结果的比较表明，最新技术能够追踪101 - 103拷贝数。
本方法能够在短时间内同时对[病原体名称]和[病原体名称]物种进行鉴别诊断和定量分析。
