Thiry L, Sprecher-Goldberger S, Vandenbussche P, Bossens M, Vereerstraten P, Hestermans-Medard O
Med Microbiol Immunol. 1979 Jan 24;165(4):255-69. doi: 10.1007/BF02152924.
Lymphocytes from a kidney grafted patient were specifically stimulated to incorporated thymidine in vitro by mitomycin C treated Hela cells infected with a Mason-Pfizer like virus (MPV). The thermolabile mutant of vesicular stomatitis virus (VSV tl) grown in leukocytes of this patient produced pseudotypes which were neutralized by the patient's sera and boy rabbit anti-MPV sera. Coculture of the patients leukocytes with SIRC cells yielded virus populations with dual properties; those of MPV plus those of another virus which was not typed serologically but possessed the biological properties of typical C particles. The patient also had neutralizing antibodies to MPV, which were detected by inhibition of syncytium formation on KC cells and by neutralization of VSV tl (MPV) pseudotypes. Preliminary results on the frequency of similar findings in other kidney grafted patients are presented.
来自肾移植患者的淋巴细胞在体外被丝裂霉素C处理过的、感染了梅森- Pfizer样病毒（MPV）的海拉细胞特异性刺激而掺入胸腺嘧啶核苷。在该患者白细胞中生长的水疱性口炎病毒（VSV tl）的热不稳定突变体产生了假型病毒，这些假型病毒被患者血清和兔抗MPV血清中和。患者白细胞与SIRC细胞共培养产生了具有双重特性的病毒群体；那些具有MPV的特性以及另一种病毒的特性，该病毒未进行血清学分型，但具有典型C颗粒的生物学特性。该患者还具有针对MPV的中和抗体，通过抑制KC细胞上的合胞体形成以及中和VSV tl（MPV）假型病毒来检测。文中呈现了其他肾移植患者中类似发现频率的初步结果。
