College of Agriculture, Anhui Science and Technology University, Fengyang, 233100, China.
College of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, China.
BMC Plant Biol. 2023 Jun 6;23(1):302. doi: 10.1186/s12870-023-04321-9.
Heat stress poses a threat to plants in arid and semiarid regions, leading to soil salinization and plant mortality. Researchers are exploring remedies to alleviate these effects, including using gibberellic acid (GA3) to regulate plant enzymes and antioxidants. Additionally, sodium nitroprusside (SNP) is gaining attention, but its combined effect with GA3 requires further research. To address this gap, we investigated the effects of GA3 and SNP on plants under heat stress conditions. For that, wheat plants were cultivated under 40 °C for 6 h per day (15 days). Sodium nitroprusside (donor of NO and SNP) and gibberellic acid (GA3), respectively, with 100 µM and 5 µg/ml concentrations, were applied as foliar sprays at 10 days after sowing (DAS). Results showed that SNP + GA3 treatment had the highest plant height (4.48% increase), plant fresh weight (29.7%), plant dry weight (87%), photosynthetic rate (39.76%) and stomatal conductance (38.10%), and Rubisco (54.2%) compared to the control. Our findings indicate a significant increase in NO, HO, TBARS, SOD, POD, APX, proline, GR, and GB that greatly scavenged reactive oxygen species (ROS) for decreasing the adverse effect of stress. Such findings confirmed the efficacy of the combined treatment of SNP + GA3 under high-temperature stress compared to the solitary application of GA3, SNP, and control. In conclusion, using SNP + GA3 is a better strategy for mitigating heat stress in wheat than individual applications. Further research is recommended to validate the effectiveness of SNP + GA3 in other cereal crops.
热应激对干旱和半干旱地区的植物构成威胁，导致土壤盐碱化和植物死亡。研究人员正在探索缓解这些影响的方法，包括使用赤霉素（GA3）来调节植物酶和抗氧化剂。此外，硝普钠（SNP）也引起了关注，但它与 GA3 的联合作用需要进一步研究。为了解决这一差距，我们研究了 GA3 和 SNP 在热应激条件下对植物的影响。为此，将小麦植株在 40°C 下每天培养 6 小时（15 天）。分别以 100µM 和 5µg/ml 浓度将硝普钠（NO 和 SNP 的供体）和赤霉素（GA3）作为叶面喷雾，在播种后 10 天（DAS）施用。结果表明，与对照相比，SNP+GA3 处理的植株高度（增加 4.48%）、植株鲜重（增加 29.7%）、植株干重（增加 87%）、光合速率（增加 39.76%）和气孔导度（增加 38.10%）以及 Rubisco（增加 54.2%）最高。与对照相比，我们发现 NO、HO、TBARS、SOD、POD、APX、脯氨酸、GR 和 GB 的含量显著增加，这些物质极大地清除了活性氧（ROS），从而降低了胁迫的不利影响。这些发现证实了 SNP+GA3 联合处理在高温胁迫下比单独使用 GA3、SNP 和对照更有效。建议进一步研究以验证 SNP+GA3 在其他谷类作物中的有效性。
