Itoh Y, Okada T S, Ide H, Eguchi G
Institute for Biophysics, Faculty of Science, University of Kyoto, Sakyo-ku, Kyoto 606, Japan.
Biological Institute, Faculty of Science, Nagoya University, Nagoya 464, Japan.
Dev Growth Differ. 1975;17(1):39-50. doi: 10.1111/j.1440-169X.1975.00039.x.
Neural retinal cells of 8-9 day-old chick embryos were differentiated into pigment cells in the conditions of cell culture for about 25 days. The increase of pigment cells in vitro was semi-quantitatively shown, by counting the number of black foci of pigmented cells per plate throughout the culture period. The increase paralleled the increase in the activity of tyrosinase. The addition of a small number of pigment cells freshly dissociated from tapeta to the cultures of neural retinae did not increase the number of black foci in vitro. Electron microscopic observations revealed the morphological differences of melanin granules between those in pigment cells of the neural retinal cultures and those in cultured tapetum cells. It was discussed that pigment cells appearing in the neural retinal cultures were derived from neural retinal cells, but not from contaminated cells of the tapetum.
8至9日龄鸡胚的神经视网膜细胞在细胞培养条件下约25天分化为色素细胞。通过在整个培养期间计数每平板色素沉着细胞的黑色斑点数量，半定量显示了体外色素细胞的增加。这种增加与酪氨酸酶活性的增加平行。将少量从绒毡层新鲜解离的色素细胞添加到神经视网膜培养物中，并未增加体外黑色斑点的数量。电子显微镜观察揭示了神经视网膜培养物色素细胞中的黑色素颗粒与培养的绒毡层细胞中的黑色素颗粒在形态上的差异。讨论认为，神经视网膜培养物中出现的色素细胞源自神经视网膜细胞，而非来自绒毡层的污染细胞。
