在眼库中使用无菌供体体层摄影术测量角膜厚度的可靠性和效率。
16 Reliability and efficiency of corneal thickness measurements using sterile donor tomography in the eye bank.
机构信息
Department of Ophthalmology, Saarland University Medical Center (UKS), Homburg/Saar, Germany.
Institute of Experimental Ophthalmology, Saarland University, Homburg/Saar, Germany.
出版信息
BMJ Open Ophthalmol. 2022 Nov;7(Suppl 2):A7. doi: 10.1136/bmjophth-2022-EEBA.16.
PURPOSE
To evaluate the reliability and efficiency of sterile corneal thickness measurements of donor corneas stored in a plastic culture flask filled with organ culture medium I (MI) or II (MII) based on tomographic data using two different software: the built-in software of the anterior segment OCT (AS-OCT) and a MATLAB self-programmed software.
METHODS
Twenty-five (25) donor corneas (50%) stored in MI and 25 (50%) in MII were imaged 5 times consecutively using an AS-OCT. The central corneal thickness (CCT) was measured both with the manual measurement tool of the AS-OCT (=CCTm) and with a MATLAB self-programmed software allowing (semi-)automated analysis (=CCTa). We analyzed the reliability of CCTm and CCTa using Cronbach´s alpha (α) and Wilcoxon signed-rank test.
RESULTS
Concerning CCTm, 68 measurements (54.4%) in MI and 46 (36.8%) in MII presented distortions in the imaged 3D-volumes and were discarded. Concerning CCTa, 5 (4%) in MI and 1 (0.8%) in MII were not analyzable. The mean (± SD) CCTm was 1129 ± 6.8 in MI and 820 ± 5.1 µm in MII. The mean CCTa was 1149 ± 2.7 and 811 ± 2.4 µm, respectively. Both methods showed a high reliability with a Cronbach´s α for CCTm of 1.0 (MI/MII) and for CCTa of 0.99 (MI) and 1.0 (MII). Nevertheless, the mean SD of the 5 measurements was significantly higher for CCTm compared to CCTa in MI (p = 0.03), but not in MII (p = 0.92).
CONCLUSIONS
Sterile donor tomography proves to be highly reliable for assessment of CCT with both methods. However, due to frequent distortions regarding the manual method, the (semi-)automated method seems to be more efficient and should be preferred.
目的
评估使用两种不同软件（眼前节 OCT 内置软件和 MATLAB 自编软件）基于断层数据对储存于充满器官培养液 I（MI）或 II（MII）的塑料培养瓶中的供体角膜进行无菌角膜厚度测量的可靠性和效率。
方法
使用眼前节 OCT 连续对 25 个储存于 MI 的供体角膜（50%）和 25 个储存于 MII 的供体角膜（50%）进行 5 次成像。使用眼前节 OCT 的手动测量工具（= CCTm）和允许（半自动）分析的 MATLAB 自编软件（= CCTa）分别测量中央角膜厚度（CCT）。我们使用 Cronbach's alpha（α）和 Wilcoxon 符号秩检验分析 CCTm 和 CCTa 的可靠性。
结果
关于 CCTm，68 次测量（54.4%）在 MI 中存在 3D 体积成像扭曲，而 46 次测量（36.8%）在 MII 中存在扭曲，因此被排除。关于 CCTa，5 次测量（4%）在 MI 中，1 次测量（0.8%）在 MII 中无法分析。MI 中的平均 CCTm 为 1129±6.8μm，MII 中的平均 CCTm 为 820±5.1μm。MI 中的平均 CCTa 为 1149±2.7μm，MII 中的平均 CCTa 为 811±2.4μm。两种方法均具有较高的可靠性，CCTm 的 Cronbach's α 为 1.0（MI/MII），CCTa 的 Cronbach's α 为 0.99（MI）和 1.0（MII）。然而，在 MI 中，CCTm 的 5 次测量的平均标准差明显高于 CCTa（p=0.03），而在 MII 中则无差异（p=0.92）。
结论
无菌供体断层摄影术可高度可靠地评估 CCT，两种方法均适用。然而，由于手动方法经常出现扭曲，因此（半自动）方法似乎更有效，应优先使用。
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