Chen Jia-Dong, Jiang Wu, Song Min-Quan, Zhou Yin-Jun, Li Ya-Ping, Duan Xiao-Jing, Tao Zheng-Ming
Zhejiang Institute of Subtropical Crops Wenzhou 325005, China.
Zhejiang Tiefengtang Biotechnology Co., Ltd. Wenzhou 325005, China.
Zhongguo Zhong Yao Za Zhi. 2023 Apr;48(7):1840-1850. doi: 10.19540/j.cnki.cjcmm.20230114.103.
Uridine diphosphate glycosyltransferase(UGT) is a highly conserved protein in plants, which usually functions in secondary metabolic pathways. This study used the Hidden Markov Model(HMM) to screen out members of UGT gene family in the whole genome of Dendrobium officinale, and 44 UGT genes were identified. Bioinformatics was used to analyze the structure, phylogeny, and promoter region components of D. officinale genes. The results showed that UGT gene family could be divided into four subfamilies, and UGT gene structure was relatively conserved in each subfamily, with nine conserved domains. The upstream promoter region of UGT gene contained a variety of cis-acting elements related to plant hormones and environmental factors, indicating that UGT gene expression may be induced by plant hormones and external environmental factors. UGT gene expression in different tissues of D. officinale was compared, and UGT gene expression was found in all parts of D. officinale. It was speculated that UGT gene played an important role in many tissues of D. officinale. Through transcriptome analysis of D. officinale mycorrhizal symbiosis environment, low temperature stress, and phosphorus deficiency stress, this study found that only one gene was up-regulated in all three conditions. The results of this study can help understand the functions of UGT gene family in Orchidaceae plants and provide a basis for further study on the molecular regulation mechanism of polysaccharide metabolism pathway in D. officinale.
尿苷二磷酸糖基转移酶（UGT）是植物中一种高度保守的蛋白质，通常在次生代谢途径中发挥作用。本研究利用隐马尔可夫模型（HMM）在铁皮石斛全基因组中筛选UGT基因家族成员，共鉴定出44个UGT基因。采用生物信息学方法对铁皮石斛UGT基因的结构、系统发育和启动子区域元件进行分析。结果表明，UGT基因家族可分为4个亚家族，各亚家族中UGT基因结构相对保守，含有9个保守结构域。UGT基因上游启动子区域含有多种与植物激素和环境因子相关的顺式作用元件，表明UGT基因表达可能受植物激素和外界环境因子诱导。比较了UGT基因在铁皮石斛不同组织中的表达情况，发现UGT基因在铁皮石斛各部位均有表达。推测UGT基因在铁皮石斛的多个组织中发挥重要作用。通过对铁皮石斛菌根共生环境、低温胁迫和缺磷胁迫的转录组分析，本研究发现只有一个基因在这三种条件下均上调表达。本研究结果有助于了解UGT基因家族在兰科植物中的功能，为进一步研究铁皮石斛多糖代谢途径的分子调控机制提供依据。
