用于产生针状光束的灵活方法及其在光学相干断层扫描中的应用。
Flexible method for generating needle-shaped beams and its application in optical coherence tomography.
作者信息
Zhao Jingjing, Winetraub Yonatan, DU Lin, VAN Vleck Aidan, Ichimura Kenzo, Huang Cheng, AAsI Sumaira Z, Sarin Kavita Y, DE LA Zerda Adam
机构信息
Department of Structural Biology, Stanford University School ofMedicine, Stanford, California 94305, USA.
Biophysics Program at Stanford, Stanford, California 94305, USA.
出版信息
Optica. 2022 Aug 20;9(8):859-867. doi: 10.1364/optica.456894. Epub 2022 Jul 22.
Abstract
Needle-shaped beams (NBs) featuring a long depth-of-focus (DOF) can drastically improve the resolution of microscopy systems. However, thus far, the implementation of a specific NB has been onerous due to the lack of a common, flexible generation method. Here we develop a spatially multiplexed phase pattern that creates many axially closely spaced foci as a universal platform for customizing various NBs, allowing flexible manipulations of beam length and diameter, uniform axial intensity, and sub-diffraction-limit beams. NBs designed via this method successfully extended the DOF of our optical coherence tomography (OCT) system. It revealed clear individual epidermal cells of the entire human epidermis, fine structures of human dermal-epidermal junction in a large depth range, and a high-resolution dynamic heartbeat of alive larvae.
摘要
具有长景深（DOF）的针状光束（NBs）可以显著提高显微镜系统的分辨率。然而，到目前为止，由于缺乏通用、灵活的生成方法，特定NB的实现一直很困难。在这里，我们开发了一种空间复用相位图案，它可以创建许多轴向紧密间隔的焦点，作为定制各种NB的通用平台，允许灵活操纵光束长度和直径、均匀轴向强度以及亚衍射极限光束。通过这种方法设计的NB成功地扩展了我们光学相干断层扫描（OCT）系统的景深。它揭示了整个人类表皮的清晰单个表皮细胞、大深度范围内人类真皮-表皮交界处的精细结构以及活幼虫的高分辨率动态心跳。
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