Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, PO Box 11103, 9300 CC Groningen, The Netherlands.
J Cell Sci. 2023 Jun 1;136(11). doi: 10.1242/jcs.259440. Epub 2023 Jun 8.
Membrane contact sites are defined as regions of close proximity between two membranes; this association is mediated by protein-protein and/or protein-lipid interactions. Contact sites are often involved in lipid transport, but also can perform other functions. Peroxisomal membrane contact sites have obtained little attention compared to those of other cell organelles. However, recent studies resulted in a big leap in our knowledge of the occurrence, composition and function of peroxisomal contact sites. Studies in yeast strongly contributed to this progress. In this Review, we present an overview of our current knowledge on peroxisomal membrane contact sites in various yeast species, including Hansenula polymorpha, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica. Yeast peroxisomes form contacts with almost all other cellular organelles and with the plasma membrane. The absence of a component of a yeast peroxisomal contact site complex results in a range of peroxisomal phenotypes, including metabolic and biogenesis defects and alterations in organelle number, size or position.
膜接触位点被定义为两个膜之间接近的区域；这种关联是由蛋白质-蛋白质和/或蛋白质-脂质相互作用介导的。接触位点通常参与脂质运输，但也可以执行其他功能。与其他细胞细胞器相比，过氧化物酶体膜接触位点受到的关注较少。然而，最近的研究使我们对过氧化物酶体接触位点的发生、组成和功能有了很大的了解。酵母的研究对此进展做出了重要贡献。在这篇综述中，我们概述了目前对各种酵母物种（包括汉逊酵母、酿酒酵母、巴斯德毕赤酵母和解脂耶氏酵母）中过氧化物酶体膜接触位点的认识。酵母过氧化物酶体与几乎所有其他细胞细胞器以及质膜形成接触。酵母过氧化物酶体接触位点复合物的一个成分缺失会导致一系列过氧化物酶体表型，包括代谢和生物发生缺陷以及细胞器数量、大小或位置的改变。
