Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, Guizhou Province, China.
Department of Genetics, University of Georgia, Athens, GA, USA.
Plant Signal Behav. 2023 Dec 31;18(1):2218670. doi: 10.1080/15592324.2023.2218670.
Adventitious roots (ARs), developing from non-root tissue, play an important role in some plants. Here, the molecular mechanism of AR differentiation in L. () with the transformed chicken interferon alpha gene (ChIFNα) encoding cytokine was studied. ChIFNα transgenic plants (TP) were identified by GUS staining, PCR, RT-PCR, and ELISA. Up to 0.175 μg/kg rChIFNα was detected in TP2 lines. Expressing rChIFNα promotes AR development by producing longer roots than controls. We found that the effect was enhanced with the auxin precursor IBA treatment in TP. IAA contents, POD, and PPO activities associated with auxin regulation were higher than wild type (WT) in TP and exogenous ChIFNα treatment plants. Transcriptome analysis revealed 48 auxin-related differentially expressed genes (DEGs) (FDR < 0.05), which expression levels were verified by RT-qPCR analysis. GO enrichment analysis of DEGs also highlighted the auxin pathway. Further analysis found that ChIFNα significantly enhanced auxin synthesis and signaling mainly with up-regulated genes of ALDH, and GH3. Our study reveals that ChIFNα can promote plant AR development by mediating auxin regulation. The findings help explore the role of ChIFNα cytokines and expand animal gene sources for the molecular breeding of growth regulation of forage plants.
不定根（ARs）从非根组织发育而来，在某些植物中起着重要作用。在这里，研究了携带鸡干扰素 alpha 基因（ChIFNα）编码细胞因子的 L.（）不定根分化的分子机制。通过 GUS 染色、PCR、RT-PCR 和 ELISA 鉴定出 ChIFNα 转基因植物（TP）。TP2 系中检测到高达 0.175μg/kg 的 rChIFNα。表达 rChIFNα比对照产生更长的根，从而促进 AR 发育。我们发现，在 TP 中，用生长素前体 IBA 处理会增强这种效果。与 WT 相比，TP 和外源 ChIFNα处理植物中的生长素调节相关的 IAA 含量、POD 和 PPO 活性更高。转录组分析揭示了 48 个与生长素相关的差异表达基因（DEGs）（FDR < 0.05），通过 RT-qPCR 分析验证了它们的表达水平。DEGs 的 GO 富集分析也突出了生长素途径。进一步分析发现，ChIFNα通过上调 ALDH 和 GH3 的基因，显著增强了生长素的合成和信号转导。我们的研究表明，ChIFNα可以通过调节生长素来促进植物 AR 的发育。这些发现有助于探索 ChIFNα 细胞因子的作用，并扩大动物基因资源，用于调控饲料植物生长的分子育种。
