A Comparative Study of Transcriptional Regulation Mechanism of Cytochrome P450 between Resistant and Susceptible Strains of .

Cytochrome P450 has previously been proved to be associated with fenvalerate-resistance in . Here, how is regulated and involved in the resistance of is studied. Seven base differences (M1-M7) were found in promoter between a fenvalerate-resistant (HDTJFR) and a susceptible (HDTJ) strain of . M1-M7 sites in HDTJFR were mutated into the corresponding base in HDTJ, and pGL3- reporter genes with different mutation sites were constructed. Fenvalerate-induced activities of reporter genes mutated at M3, M4, and M7 sites were significantly reduced. Transcription factors Ubx and Br, whose binding sites contain M3 and M7, respectively, were overexpressed in HDTJFR. Knockdown of and results in significant expression inhibition of and other resistance-related P450 genes, and increase of sensitivity of to fenvalerate. These results indicate that Ubx and Br regulate the expression of to mediate the fenvalerate-resistance in .