Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
J Agric Food Chem. 2023 Jun 21;71(24):9314-9323. doi: 10.1021/acs.jafc.3c01593. Epub 2023 Jun 8.
Cytochrome P450 has previously been proved to be associated with fenvalerate-resistance in . Here, how is regulated and involved in the resistance of is studied. Seven base differences (M1-M7) were found in promoter between a fenvalerate-resistant (HDTJFR) and a susceptible (HDTJ) strain of . M1-M7 sites in HDTJFR were mutated into the corresponding base in HDTJ, and pGL3- reporter genes with different mutation sites were constructed. Fenvalerate-induced activities of reporter genes mutated at M3, M4, and M7 sites were significantly reduced. Transcription factors Ubx and Br, whose binding sites contain M3 and M7, respectively, were overexpressed in HDTJFR. Knockdown of and results in significant expression inhibition of and other resistance-related P450 genes, and increase of sensitivity of to fenvalerate. These results indicate that Ubx and Br regulate the expression of to mediate the fenvalerate-resistance in .
细胞色素 P450 先前已被证明与氰戊菊酯抗性有关。在这里，研究了 如何被调控以及如何参与 的抗性。在对氰戊菊酯具有抗性的（HDTJFR）和敏感的（HDTJ） 种群之间，发现 启动子中有 7 个碱基差异（M1-M7）。将 HTDJFR 中的 M1-M7 位点突变为 HTDJ 中的相应碱基，并构建了具有不同突变位点的 pGL3-报告基因。突变 M3、M4 和 M7 位点的报告基因的氰戊菊酯诱导活性显著降低。分别含有 M3 和 M7 的结合位点的转录因子 Ubx 和 Br 在 HTDJFR 中过表达。 和 的敲低导致 和其他抗性相关 P450 基因的表达显著抑制，以及 对氰戊菊酯的敏感性增加。这些结果表明 Ubx 和 Br 调节 的表达，以介导 在氰戊菊酯中的抗性。
