Harbor Branch Oceanographic Institute, Florida Atlantic University, 5600 US 1 N, Fort Pierce, FL 34946, United States of America.
Harbor Branch Oceanographic Institute, Florida Atlantic University, 5600 US 1 N, Fort Pierce, FL 34946, United States of America.
Harmful Algae. 2023 Jul;126:102437. doi: 10.1016/j.hal.2023.102437. Epub 2023 Apr 30.
The Indian River Lagoon (IRL) spans approximately one-third of the east coast of Florida and, in recent years, has faced frequent harmful algal blooms (HABs). Blooms of the potentially toxic diatom, Pseudo-nitzschia, occur throughout the lagoon and were reported primarily from the northern IRL. The goal of this study was to identify species of Pseudo-nitzschia and characterize their bloom dynamics in the southern IRL system where monitoring has been less frequent. Surface water samples collected from five locations between October 2018 and May 2020 had Pseudo-nitzschia spp. present in 87% of samples at cell concentrations up to 1.9×10 cells mL. Concurrent environmental data showed Pseudo-nitzschia spp. were associated with relatively high salinity waters and cool temperatures. Six species of Pseudo-nitzschia were isolated, cultured, and characterized through 18S Sanger sequencing and scanning electron microscopy. All isolates demonstrated toxicity and domoic acid (DA) was present in 47% of surface water samples. We report the first known occurrence of P. micropora and P. fraudulenta in the IRL, and the first known DA production from P. micropora.
印度河泻湖 (IRL) 横跨佛罗里达州东海岸的大约三分之一，近年来，经常发生有害藻类大量繁殖 (HABs)。潜在毒性硅藻 Pseudonitzschia 的大量繁殖发生在泻湖的整个区域，主要报告来自泻湖的北部地区。本研究的目的是鉴定 Pseudo-nitzschia 的物种，并描述它们在监测较少的南部 IRL 系统中的繁殖动态。从 2018 年 10 月至 2020 年 5 月期间从五个地点采集的地表水样本中，高达 1.9×10 细胞 mL 的细胞浓度中，87%的样本中存在 Pseudo-nitzschia spp。同时的环境数据表明，Pseudo-nitzschia spp. 与相对高盐度水和低温有关。通过 18S Sanger 测序和扫描电子显微镜共分离、培养和鉴定了六种 Pseudo-nitzschia 。所有分离株均表现出毒性，47%的地表水样本中存在 DA。我们报告了在 IRL 中首次发现 P. micropora 和 P. fraudulenta，以及首次从 P. micropora 中检测到 DA 产生。
